基于蛋白质组学技术的鹿茸不同部位差异蛋白筛选

发布时间：2018-04-26 16:21

本文选题：梅花鹿 + 鹿茸　；参考：《畜牧兽医学报》2017年04期

【摘要】：旨在了解梅花鹿鹿茸不同部位蛋白表达差异,本研究以腊片部位、血片部位和骨片部位的梅花鹿鹿茸组织为试验材料,运用双向电泳(2-DE)和MALDI-TOF-TOF串联质谱技术对不同部位梅花鹿鹿茸差异表达蛋白质进行鉴定,并对差异蛋白进行生物信息学分析。结果表明,成功筛选出37个差异表达蛋白,主要涉及多细胞生物体发生、解毒、细胞成分组织或生物发生、细胞聚集、定位、对刺激的反应、生物黏附、发育、单一生物体发生、免疫系统等生物学过程。其中鹿茸腊片、血片、骨片组织中分别有18、5与14个蛋白质表达上调。对差异表达蛋白作进一步分析发现,转录延伸因子(EFB1)、视黄酸结合蛋白(CRABP1)在鹿茸的快速生长中起重要调控作用,而载脂蛋白A1(APOA1)、脂肪酸结合蛋白4(FABP4)、转甲状腺素蛋白(TTR)在鹿茸的快速骨化中起重要的调控作用,对鹿茸快速生长与骨化机制的进一步研究具有重要意义。
[Abstract]:In order to understand the difference of protein expression in different parts of deer antler of sika deer, the tissue of deer antler of sika deer was used as experimental material. Two-dimensional electrophoresis (2-DE) and MALDI-TOF-TOF tandem mass spectrometry were used to identify the differentially expressed proteins in deer antler of sika deer, and the differential proteins were analyzed by bioinformatics. The results showed that 37 differentially expressed proteins were successfully screened, mainly involved in the occurrence of multicellular organisms, detoxification, cell component tissue or biogenesis, cell aggregation, localization, response to stimulation, biological adhesion, development, Biological processes such as the occurrence of a single organism, the immune system, etc. Among them, 1855 and 14 proteins were upregulated in pilose velvet slices, blood slices and bone slices, respectively. Further analysis of differentially expressed proteins revealed that the transcription extension factor EFB1 and retinoic acid binding protein CRABP1 play an important role in the rapid growth of velvet antler. Apolipoprotein A1C APOA1, fatty acid binding protein 4FABP4 and TTRs play an important role in the rapid ossification of velvet antler, and play an important role in the further study of the mechanism of rapid growth and ossification of velvet antler.
【作者单位】：中国农业科学院特产研究所特种经济动物分子生物重点实验室;
【基金】：基金项目:特种动物遗传资源创新团队(CAAS-ASTIP-201X-ISAPS)
【分类号】：S825.2

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