高致病性猪繁殖与呼吸综合征病毒TJM-F92疫苗株对猪瘟兔化弱毒株的间隔免疫干扰研究

发布时间：2018-04-27 00:25

本文选题：PRRSV + TJM-F株　；参考：《中国预防兽医学报》2017年07期

【摘要】：为研究高致病性猪繁殖与呼吸综合征病毒(PRRSV)TJM-F92疫苗株对猪瘟病毒(CSFV)C株的间隔免疫干扰情况,本实验设计在免疫高剂量PRRSV TJM-F92株疫苗毒后第7 d和第14 d分别免疫低剂量CSFV C株疫苗毒,并于CSFV C株疫苗毒免疫后第14 d用CSFV石门系血毒攻击实验动物,通过体液免疫水平、免疫攻毒保护水平及病理学变化等方面进行评价。结果表明,间隔7 d免疫组与间隔14 d免疫组CSFV ELISA抗体水平与CSFV C株单独免疫对照组抗体水平无显著差异;攻击CSFV石门系血毒后,间隔7 d免疫组与间隔14 d免疫组均未出现猪瘟临床症状,组织器官病理变化轻微,对CSFV石门系血毒攻击可产生良好的保护效果。CSF阴性对照组实验动物5/5表现出猪瘟明显临床症状并全部死亡。以上结果表明,高剂量PRRSV TJM-F92疫苗株与低剂量CSFV C株间隔7 d或14 d免疫,PRRSV TJM-F92株对CSFV C株无免疫干扰作用。
[Abstract]:In order to study the interval immune interference of highly pathogenic porcine reproductive and respiratory syndrome virus (PRRSVV) TJM-F92 vaccine strain against CSFV strain, In this experiment, low dose PRRSV TJM-F92 C vaccine was immunized on the 7th and 14th day after immunization with high dose PRRSV TJM-F92 strain vaccine, and the experimental animals were attacked with CSFV stone portal blood virus on the 14th day after CSFV C vaccine vaccination, and the humoral immunity level was passed. The protective level and pathological changes were evaluated. The results showed that there was no significant difference in the level of CSFV ELISA antibody between the immunized group and the control group after 7 days and 14 days, and the antibody level of the control group immunized with CSFV C strain was not significantly different from that of the control group. There were no clinical symptoms of CSFV in the immunization group of 7 days and 14 days, and the pathological changes of tissues and organs were slight. 5 / 5 of the experimental animals in the CSF-negative control group showed obvious clinical symptoms of CSFV and all of them died. The results indicated that the high dose PRRSV TJM-F92 vaccine strain and the low dose CSFV C strain had no immune interference effect on CSFV C strain after 7 or 14 days of immunization.
【作者单位】：中国农业科学院特产研究所;华威特(北京)生物科技有限公司;中国农业大学生物学院;中国兽医药品监察所;
【基金】：基金项目:新型重大动物疫病疫苗与诊断试剂创制及生产工艺创新(2011AA10A213)
【分类号】：S852.651

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