中国西门塔尔牛PPP1CB和ACSL4基因多态性及与屠宰性状的相关分析

发布时间：2018-04-27 00:36

本文选题：西门塔尔牛 + PPP1CB　；参考：《吉林大学》2015年硕士论文

【摘要】：西门塔尔牛广泛分布于中国各地，其肉质细嫩，大理石花纹明显，口感好，以其优质的乳肉兼用性，良好的生产性状已经发展成为大型乳肉兼用新品种。通过测定共获得了259头育肥的西门塔尔牛屠宰性状的相关数据，这些数据为后期利用基因工程方法筛选功能基因以及全基因组关联，建立基因聚合育种方法和分子标记辅助选择等提供基础，更为中国西门塔尔牛的早期选种选育和遗传改良提供理论依据。 PPP1CB基因编码的蛋白质是蛋白磷酸酶1催化亚基之一，蛋白磷酸酶1是主要的丝-苏氨酸蛋白磷酸酶,在细胞中能够普遍表达，蛋白激酶和蛋白磷酸酶协同作用能够使蛋白质磷酸化与去磷酸化，调节众多生理活动：如细胞内糖代谢、细胞周期、肌肉收缩等。哺乳动物长链脂酰CoA合成酶（ACSL）能够使脂肪酸活化，是哺乳动物体利用脂肪酸的第一步，对于磷脂合成，脂肪酸分解，和细胞膜重构等过程是极其重要的。PPP1CB基因和ACSL4基因多态性与牛屠宰性状相关性的研究国内外鲜有报道，作为在本课题组前期基因芯片分析结果中的差异表达基因，本研究将PPP1CB基因和ACSL4基因作为西门塔尔牛屠宰性状的候选基因。本研究的目的在于寻找PPP1CB和ACSL4基因的多态性位点与西门塔尔牛屠宰性状之间的相关性，以259头中国西门塔尔牛草原型为研究对象，并收集其生产数据及屠宰数据，利用PCR-RFLP技术检测134头中国西门塔尔牛PPP1CB基因和259头西门塔尔牛ACSL4基因上的SNP位点，并利用统计学分析的方法，对PPP1CB和ACSL4基因的多态性位点与中国西门塔尔牛屠宰性状之间的相关性进行分析。结果显示，西门塔尔牛PPP1CB基因的内含子1第14434个碱基处存在C→T的单碱基突变，PPP1CB基因内含子1不同基因型与西门塔尔牛屠宰性状的屠宰率存在显著相关性，，CT型的屠宰率显著高于TT型（P0.05），其他性状在不同基因型间的差异不显著。ACSL4基因的内含子10第2327个碱基处存在G→C的单碱基突变，ACSL4基因内含子10的不同基因型与西门塔尔牛屠宰性状无显著相关性。
[Abstract]:Simmental cattle are widely distributed in all parts of China, its meat quality is tender, marbling pattern is obvious, taste is good, because of its high quality milk and meat use, good production character has developed into a large new variety for both milk and meat. Data on slaughter traits of 259 fattening Simmental cattle were obtained. These data were used to screen functional genes and the whole genome association by genetic engineering method. The establishment of gene aggregation breeding methods and molecular marker-assisted selection provides a theoretical basis for early selection and genetic improvement of Simmental cattle in China. The protein encoded by PPP1CB gene is one of the catalytic subunits of protein phosphatase 1. Protein phosphatase 1 is the main serine threonine protein phosphatase, which can be widely expressed in cells. The synergistic action of protein kinase and protein phosphatase can make protein phosphorylation and dephosphorylation regulate many physiological activities such as intracellular glucose metabolism cell cycle muscle contraction and so on. Mammal long chain lipoyl CoA synthase (CoA) can activate fatty acids, which is the first step in the utilization of fatty acids in mammals. For phospholipid synthesis, fatty acids are decomposed. The correlation between the polymorphism of the .PPP1CB gene and the ACSL4 gene and the slaughter traits of cattle was rarely reported at home and abroad, which was used as the differentially expressed gene in the results of gene chip analysis in the early stage of our research group. In this study, PPP1CB gene and ACSL4 gene were used as candidate genes for slaughter traits of Simmental cattle. The purpose of this study was to find out the association between the polymorphism of PPP1CB and ACSL4 gene and the slaughter traits of Simmental cattle. 259 Chinese Simmental cattle were selected as the study subjects, and their production data and slaughter data were collected. The SNP loci of PPP1CB gene and ACSL4 gene in 134 Chinese Simmental cattle and 259 Simmental cattle were detected by PCR-RFLP technique. The relationship between the polymorphism of PPP1CB and ACSL4 genes and slaughter traits of Simmental cattle in China was analyzed. The results show that In Simmental cattle, there is a significant correlation between different genotypes of intron 1 of PPP1CB gene and slaughter rate of slaughtering traits of Simmental cattle. There is a significant correlation between different genotypes of intron 1 and the slaughter rate of CT-type in Simmental cattle. ACSL4 gene had no significant difference among different genotypes. At 2327 base of intron 10 of ACSL4 gene, there were different genotypes of single base mutation ACSL4 gene and slaughterability of Simmental cattle. There was no significant correlation.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S823

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