肠炎沙门菌非编码小RNA RyhB-1和IsrE对清远麻鸡的致病性

发布时间：2018-04-27 04:11

本文选题：肠炎沙门菌 + ryhB-　；参考：《中国兽医学报》2017年09期

【摘要】：RyhB是一种大小为90bp左右的细菌非编码小RNA,已有研究表明存在于鼠伤寒沙门菌和霍乱弧菌中的2种RyhB同源物RyhB-1和IsrE可调控细菌生物膜形成、趋化性和耐酸性。为研究RyhB在肠炎沙门菌致病性上的作用,以及2种同源物能否对毒力调控发挥协同作用,本试验利用λ-Red同源重组系统构建了ryhB-1和isrE单、双缺失株,利用表达载体pBR322和pACYC184分别构建了回补质粒并导入缺失株50336△ryhB-1,50336△isrE,50336△ryhB-1/△isrE中,获得各突变株相应的回补菌株,检测了野生株和各突变株对1日龄雏鸡半数致死量(LD50)、致死率、体内分布等致病性差异。结果发现,ryhB-1和isrE基因缺失后导致肠炎沙门菌毒力减弱,双基因缺失株毒力下降更为明显,表明2种小RNA均对肠炎沙门菌致病性具有增强作用,且二者对毒力的调控具有协同作用。
[Abstract]:RyhB is a small bacterial noncoding RNAs about the size of 90bp. Two RyhB congeners, RyhB-1 and IsrE, which exist in Salmonella typhimurium and Vibrio cholerae, have been shown to regulate bacterial biofilm formation, chemotaxis and acid tolerance. In order to study the role of RyhB in pathogenicity of Salmonella enteritis and whether the two congeners can exert synergistic effect on virulence regulation, single and double deletion strains of ryhB-1 and isrE were constructed by 位 -Red homologous recombination system. The complementary plasmids were constructed by using the expression vector pBR322 and pACYC184, respectively, and were introduced into the missing strain 50336 ryhB-1 / 50336isEN 50336 ryhB-1/ isrE. The corresponding backfill strains were obtained. The half lethal dose of LD50 and the lethal rate of the wild strains and the mutant strains to 1-day-old chicks were detected. The pathogenicity difference of distribution in vivo. The results showed that the virulence of Salmonella enteritidis was decreased after the deletion of ryhB-1 and isrE genes, and the virulence of the double gene deletion strain was more obvious. The results showed that the two kinds of small RNA could enhance the pathogenicity of Salmonella enteritis. And they have synergistic effect on virulence regulation.
【作者单位】：扬州大学兽医学院;江苏省动物重要疫病与人兽共患病防控协同创新中心;江苏农牧科技职业学院;江苏省家禽科学研究所;
【基金】：江苏省高校自然科学研究资助项目(14KJB230002) 兽医生物技术国家重点实验室开放课题资助项目(SKLVBF201509) 扬州市自然科学基金青年科技人才资助项目(YZ2014019) 科技部畜禽重大疫病防控与高效安全养殖综合技术研发重点专项资助项目(2016YFD0500900) 江苏高校优势学科建设工程资助项目(PAPD) 国家自然科学基金资助项目(31101826,31270171) 泰州市农业科技发展资助项目(TZ201422)
【分类号】：S858.31

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