饲用产甘露聚糖酶芽孢杆菌的筛

发布时间：2018-04-27 21:05

本文选题：芽孢杆菌 + 甘露聚糖酶　；参考：《中国畜牧兽医》2017年08期

【摘要】：为了筛选一株产甘露聚糖酶的芽孢杆菌,并考察其对肉鹅生长性能的影响,本试验采用刚果红平板变色圈法进行产甘露聚糖酶芽孢杆菌的初筛,通过液体发酵测定酶活力的方法展开复筛,并进行动物安全性和胃肠道耐受性试验。通过形态特征观察、生理生化试验和16SrDNA全序列分析与发育树构建将其鉴定到种。制备该菌菌剂,并将其按0.10%、0.25%、0.50%、0.75%和1.00%添加于肉鹅日粮中,考察其对肉鹅日增重和料重比的影响。结果表明:筛选到产甘露聚糖酶的菌株枯草芽孢杆菌(Bacillus subtils)Y-3-2,其产酶活力为45.939U/mL,耐受pH 2.0,在0.1%的胆酸盐溶液和人工肠液中可正常生长。将其制成菌剂,在肉鹅日粮中按0.25%添加,可使试验组肉鹅日增重较对照组提高8.78%(P0.01),料重比降低6.69%(P0.01)。以上结果说明在肉鹅日粮中添加本试验筛选出的产甘露聚糖酶芽孢杆菌可提高肉鹅的饲料利用率,对肉鹅的生长有显著促进作用。
[Abstract]:In order to screen a Bacillus producing manna producing polyglycase and investigate its effect on the growth performance of meat goose, this experiment uses the Congo red plate discoloration ring method to screen the Bacillus mannanase bacillus and resieve the enzyme activity by liquid fermentation, and carry out the animal safety and gastrointestinal tolerance test. State characteristics observation, physiological and biochemical tests and 16SrDNA full sequence analysis and development tree construction were used to identify the species. The bacteria were prepared and added to the goose diet by 0.10%, 0.25%, 0.50%, 0.75% and 1%, and the effects on the daily gain and feed weight ratio of meat goose were investigated. The results showed that Bacillus subtilis producing manna producing strain was screened. (Bacillus subtils) Y-3-2, its enzyme activity is 45.939U/mL, tolerated pH 2, and can grow normally in 0.1% bile acid salt solution and artificial intestinal liquid. It is made into a fungus agent and added in the meat goose diet by 0.25%, which can increase the daily gain of the goose in the test group by 8.78% (P0.01) and the weight ratio of 6.69% (P0.01). The above results show that the goose diet is in the meat diet. The addition of mannase producing Bacillus can improve the feed utilization rate of goose and promote the growth of goose.

【作者单位】：河北农业大学生命科学学院;
【基金】：河北省教育厅科学技术研究项目(2011232)
【分类号】：S835.5

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