仔猪腹泻大肠杆菌流行株鉴定及毒力与耐药性分析

发布时间：2018-04-28 13:11

本文选题：新生仔猪 + 腹泻　；参考：《中国畜牧兽医》2017年04期

【摘要】：为明确河南地区一规模化猪场暴发新生仔猪腹泻疫情的病原特性及其耐药谱,本研究对肠道致病大肠杆菌进行了分离鉴定。对腹泻仔猪粪便中分离的大肠杆菌进行13种毒力基因检测和21种抗生素的药敏试验。结果显示,34.8%分离株检出EAST1基因;所获分离株均为多重耐药菌株,且至少对5类抗生素耐药,耐9类以上抗生素的菌株达到75.3%,对氨苄西林、哌拉西林、环丙沙星、左氧氟沙星和莫西沙星耐药率达到100.0%。细菌种系进化分群结果显示,21.7%分离株属于有致病性的B2群和D群。本研究获得了腹泻仔猪大肠杆菌分离株的毒力基因、耐药谱的基本数据和种系进化分群的遗传背景,为制定有针对性的治疗和防控技术方案提供依据。
[Abstract]:In order to identify the pathogenic characteristics and drug resistance spectrum of diarrhea outbreak in a large scale pig farm in Henan Province, the pathogenic Escherichia coli of intestinal tract was isolated and identified in this study. 13 virulence genes and 21 antibiotics were tested for Escherichia coli isolated from feces of piglets with diarrhea. The results showed that the EAST1 gene was detected in 34.8% of the isolates, and all the isolates were multidrug resistant, and were resistant to at least 5 kinds of antibiotics. The strains resistant to more than 9 classes of antibiotics were 75.3%, and were resistant to ampicillin, piperacillin, ciprofloxacin, ampicillin, piperacillin and ciprofloxacin. The drug resistance rates of levofloxacin and moxifloxacin were 100.0. The results of phylogeny showed that 21.7% of the isolates belonged to B _ 2 and D groups with pathogenicity. In this study, the virulence genes, the basic data of drug resistance spectrum and the genetic background of phylogenetic evolution of Escherichia coli isolates from piglets with diarrhea were obtained, which provided the basis for the formulation of targeted treatment and control techniques.
【作者单位】：吉林大学动物医学学院;军事医学科学院军事兽医研究所吉林省人兽共患病预防与控制重点实验室;
【基金】：吉林省自然科学基金项目(20150101110JC) 国家科技重大专项(2013ZX10004-217)
【分类号】：S858.28

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