4个主效基因与英系长白猪繁殖性能的相关性分析

发布时间：2018-04-28 23:15

本文选题：英系长白猪 + 主效基因　；参考：《中国畜牧兽医》2017年04期

【摘要】：为寻找有效的分子标记用于英系长白猪辅助育种进而提高其繁殖性能,在英系长白猪群(169头)中采用PCR-RFLP技术,进行雌激素受体(estrogen receptor,ESR)、视黄醛结合蛋白(retinol binding protein 4,RBP4)、瘦素(leptin,LEP)和促卵泡素亚基β(follicle-stimulating hormoneβ,FSHβ)4种与繁殖性状相关基因的基因型频率检测,利用SPSS 19.0统计软件,采用最小二乘法将各基因不同基因型与总产仔数、产活仔数、断奶窝重、断奶头数进行了相关分析。结果显示,ESR、RBP4、LEP基因均以A为优势等位基因,FSHβ基因以B为优势等位基因,其中LEP、FSHβ基因多态位点符合Hardy-Weinberg平衡定律(P0.05),而ESR、RBP4基因多态位点处于Hardy-Weinberg不平衡状态(P0.01);LEP基因在长白猪母猪中表现为BB基因型为有利基因型,其总产仔数、产活仔数、断奶头数分别比AB基因型高1.3219、1.6855、1.1710头(P0.05);FSHβ基因中AA基因型为不利基因型,BB基因型断奶窝重比AA基因型高5.1129kg(P0.05);ESR、RBP4基因对长白母猪繁殖性能均无显著影响(P0.05),而LEP、FSHβ基因可能作为有价值的候选基因应用于英系长白猪的分子标记辅助选择。
[Abstract]:In order to find effective molecular markers for auxiliary breeding of British Landrace pigs and improve their reproductive performance, PCR-RFLP technique was used in 169 British Landrace pigs. The genotypic frequencies of four genes associated with reproductive traits, including estrogen receptor (ER), retinol binding protein 4 (RBP4) and follicle-stimulating subunit (尾 follicle-stimulating hormone 尾 -FSH 尾), were detected by SPSS 19.0 software. The correlation between different genotypes of genes and total litter size, live litter size, weaning litter weight and weaning head number were analyzed by least square method. The results showed that A was the dominant allele and FSH 尾 gene was the dominant allele B in the RBP4LEP gene of ESRS. The polymorphic loci of LEP- FSH 尾 gene were in accordance with the Hardy-Weinberg equilibrium law (P0.05), while the polymorphic loci of ESR-RBP4 gene were in the state of Hardy-Weinberg disequilibrium. In Landrace sows, the genotype of BB was a favorable genotype, and the total litter size and the number of live litter were all favorable. The number of weanling heads was 1.3219 / 1.6855 / 1.1710 higher than that of AB genotype, respectively. The weaning litter weight of AA genotype and BB genotype was 5.1129 kg / kg RBP4 gene higher than that of AA genotype, respectively, and there was no significant effect on reproductive performance of Landrace sows, while LEPFSH 尾 gene might be used as a marker for the reproductive performance of Landrace sows. The valuable candidate genes were applied to the molecular marker assisted selection of British Landrace pigs.
【作者单位】：河北农业大学动物科技学院;国家北方山区农业工程技术研究中心;河北省山区农业工程技术研究中心;河北工程大学生命科学与食品工程学院;河北双鸽美丹畜牧科技有限公司;河北省畜牧良种工作站;
【基金】：河北省现代农业产业技术体系专项(HBCT2013070202)
【分类号】：S828

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