诱导型腹水综合征肉鸡心肌的病理变化与增殖

发布时间：2018-04-28 23:10

本文选题：腹水综合征 + 肉鸡　；参考：《中国兽医科学》2017年08期

【摘要】：为探索诱导型腹水综合征肉鸡心脏的病理变化与心肌细胞增殖之间的关系,将20日龄白羽肉鸡分成对照组(静脉注射肝素钠生理盐水,n=30)和试验组(静脉注射含纤维素微粒的肝素钠生理盐水,n=90)。自注射纤维素微粒后,记录腹水综合征(AS)发病率,并于肉鸡42日龄时取样,测定右心室重量与全心室重量之比(mrv/mtv)、红细胞压积(PCV)以及血清中谷草转氨酶(AST)活性浓度和肌酸激酶(CK)活性浓度;右心室组织常规苏木精—伊红染色,并运用实时荧光定量PCR测定右心室组织PCNA和Ki-67的表达量。结果,与对照组相比,试验组肉鸡AS发病率、mrv/mtv值、PCV及血清中AST活性浓度和CK活性浓度显著增高(P0.01);试验组肉鸡的右心室明显扩张,心肌细胞出现水肿现象,但PCNA和Ki-67的m RNA表达量无明显变化。上述结果表明,诱导型腹水综合征肉鸡心脏产生了严重的病理变化,但心肌所产生的这种病变与细胞的增殖无关。
[Abstract]:To explore the relationship between cardiac pathological changes and cardiomyocyte proliferation in broilers with induced ascites syndrome. White broilers of 20 days old were divided into two groups: control group (intravenous injection of heparin sodium normal saline) and experimental group (intravenous injection of sodium heparin sodium saline containing cellulose particles). The incidence of ascitic syndrome (ASS) was recorded after injecting cellulose particles and was sampled at 42 days of age in broilers. The ratio of right ventricular weight to whole ventricular weight (RV / W), hematocrit (PCV), aspartate aminotransferase (AST) activity and creatine kinase (CK) activity in serum were measured, and the right ventricular tissue was stained with hematoxylin and eosin. The expression of PCNA and Ki-67 in right ventricular tissue was measured by real-time fluorescence quantitative PCR. The results showed that compared with the control group, the incidence of as in the experimental group was significantly higher than that in the control group, and the AST activity and CK activity concentration in serum were significantly higher than those in the control group, while the right ventricle was dilated and the cardiomyocyte edema was observed in the experimental group. However, the expression of m RNA in PCNA and Ki-67 did not change significantly. These results indicated that the heart of broiler with induced ascites syndrome had serious pathological changes, but the pathological changes of myocardium were not related to cell proliferation.
【作者单位】：华南农业大学兽医学院;
【基金】：国家自然科学基金项目(31502131) 十三五国家重点研发计划项目(2016YFD0501205)
【分类号】：S858.31

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