紫薯花色苷对小鼠脾淋巴细胞免疫调节活性的研究

发布时间：2018-04-29 22:27

本文选题：紫薯花色苷 + 淋巴细胞　；参考：《锦州医科大学》2017年硕士论文

【摘要】：目的为探讨紫薯花色苷(Purple Sweet Potato Anthocyanins,PSPA)的免疫调节作用,本文建立以原代培养的小鼠脾淋巴细胞为靶细胞的PSPA免疫调节功能模型,体外研究PSPA的免疫调节活性,为PSPA的开发和利用提供实验依据。方法本文以体外建立的小鼠脾淋巴细胞为靶细胞的PSPA免疫调节功能模型,采用MTT法检测PSPA组、PSPA与刀豆蛋白A(Con A)或脂多糖(LPS)复合药组对淋巴细胞增殖及PSPA对金黄色葡萄球菌感染淋巴细胞增殖的影响;采用ELISA法检测PSPA对淋巴细胞分泌细胞因子及对金黄色葡萄球菌感染淋巴细胞分泌细胞因子的影响;采用RT-PCR法检测PSPA对淋巴细胞分泌细胞因子基因IL-2、IL-10、IL-13和TNF-αm RNA的表达水平。结果PSPA组、PSPA与Con A或LPS复合药组刺激淋巴细胞,均能提高淋巴细胞的刺激指数,且PSPA与Con A或LPS复合药组刺激淋巴细胞指数均高于PSPA组;在金黄色葡萄球菌感染时,PSPA组刺激淋巴细胞,能提高淋巴细胞的刺激指数,但低于未感染金黄色葡萄球菌时的刺激指数;PSPA组刺激淋巴细胞,能诱导淋巴细胞IL-2、IL-10、IL-13和TNF-α的分泌,但在金黄色葡萄球菌感染时,诱导淋巴细胞IL-2、IL-10、IL-13和TNF-α的分泌均低于未感染金黄色葡萄球菌时的分泌;PSPA能上调淋巴细胞分泌细胞因子基因IL-2、IL-10、IL-13和TNF-αm RNA的表达水平。结论在一定浓度范围内(PSPA为2.5、5、10、20 mg/m L),PSPA组、PSPA与Con A或LPS复合药组刺激淋巴细胞,均能促进淋巴细胞的增殖,且PSPA与Con A或LPS复合药组能发挥协同作用;在金黄色葡萄球菌感染淋巴细胞时,PSPA组刺激淋巴细胞增殖能力低于未感染组。PSPA组刺激淋巴细胞及对金黄色葡萄球菌感染淋巴细胞时,能诱导淋巴细胞因子IL-2、IL-10、IL-13和TNF-α的分泌;PSPA通过诱导Th1和Th2型细胞因子的分泌,上调细胞因子基因IL-2、IL-10、IL-13和TNF-αm RNA的表达水平,从而提高淋巴细胞的免疫调节活性。
[Abstract]:Objective to investigate the immunomodulatory effect of Purple Sweet Potato anthocyanins (PSPA) in vitro, a model of PSPA immunomodulatory function was established using primary cultured mouse spleen lymphocytes as target cells, and the immunomodulatory activity of PSPA was studied in vitro. To provide experimental basis for the development and utilization of PSPA. Methods PSPA immunomodulatory function model was established with mouse spleen lymphocytes as target cells in vitro. MTT method was used to detect the effect of PSPA group combined with concanavalin A(Con A or lipopolysaccharide (LPS) on lymphocyte proliferation and PSPA on lymphocyte proliferation of Staphylococcus aureus infection. The effects of PSPA on cytokines secreted by lymphocytes and on cytokines secreted by lymphocytes in Staphylococcus aureus infection were detected by ELISA method, and the expression of IL-2mIL-10 IL-13 and TNF- 伪 m RNA by PSPA was detected by RT-PCR method. Results the lymphocyte stimulating index of PSPA group was higher than that of PSPA group, and that of PSPA combined with Con A or LPS group was higher than that of PSPA group. The stimulation of lymphocytes in the PSPA group was lower than that in the non-infected Staphylococcus aureus group, but it could induce the secretion of IL-2, IL-10, IL-13 and TNF- 伪 in the lymphocytes, but the stimulation index of the PSPA group was lower than that of the non-infected Staphylococcus aureus group, and the stimulation index of the PSPA group was lower than that of the non-infected Staphylococcus aureus group. But in Staphylococcus aureus infection, the secretion of IL-10 IL-13 and TNF- 伪 in lymphocytes induced by Staphylococcus aureus was lower than that in uninfected Staphylococcus aureus. PSPA could up-regulate the expression of IL-13 and TNF- 伪 m RNA. Conclusion in a certain concentration range, the proliferation of lymphocytes can be stimulated by the combination of PSPA and Con A or LPS in the PSPA group with a range of 2.5 ~ 5 ~ 10 ~ 10 mg/m / L ~ 20 mg/m / L, and the synergistic effect of PSPA and Con A or LPS compound group can be achieved. When Staphylococcus aureus infected lymphocytes, the ability of stimulating lymphocyte proliferation in PSPA group was lower than that in uninfected group. It can induce the secretion of IL-13 and TNF- 伪 by inducing the secretion of Th1 and Th2 cytokines, and up-regulate the expression of IL-13 and TNF- 伪 m RNA, thus enhancing the immunomodulatory activity of lymphocytes.
【学位授予单位】：锦州医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S853.74

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