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荣昌母猪妊娠期标准回肠可消化赖氨酸适宜需要量研究

发布时间:2018-04-30 02:41

  本文选题:荣昌母猪 + SID ; 参考:《西南大学》2016年硕士论文


【摘要】:本研究将母猪妊娠期分成妊娠1~90d和妊娠91~110d两个阶段,通过两个试验来研究荣昌母猪妊娠期日粮适宜标准回肠可消化赖氨酸的需要量。试验一:为研究荣昌初产母猪妊娠1~90d日粮标准回肠可消化赖氨酸的需要,选取50头后备荣昌母猪按体重随机分为5个处理,每个处理10头,饲喂SID Lys水平分别为0.35%、0.45%、0.55%、0.65%、0.75%,代谢能水平为11.75MJ/kg的日粮;母猪从妊娠91d开始到哺乳期结束饲喂相同日粮。试验期从母猪配种至仔猪断奶,分别在母猪配种时、妊娠90d、分娩时(分娩后24h内)、断奶时测定母猪的体重与背膘厚,母猪分娩时记录产仔数、产活仔数、仔猪初生重等,测定母猪妊娠90d时的血糖、血清总蛋白、血清尿素氮、胰岛素、类胰岛素样生长因子-I、孕酮、催乳素以及哺乳期仔猪的血糖、总蛋白、尿素氮。结果表明:母猪妊娠1~90d体重及背膘厚随日粮SID Lys增加各处理组在数值上呈现先升高后下降的趋势,但没有达到显著水平(P0.05);妊娠90d各处理组血清总蛋白、血糖、甘油三酯、总胆固醇的值随着日粮SID Lys水平的增加呈现出先升高后下降的趋势,但没有达到显著水平(P0.05)。妊娠90d各处理组血清尿素氮含量随着日粮SID Lys水平的增加呈现出先下降后上升的趋势,且0.55%组和0.65%组显著低于0.35%组(0.01P0.05),妊娠90d血清尿素氮含量(Y)与日粮SID Lys水平(X)之间具有二次曲线的关系:Y=12.571X2-14.889X+6.9424(R2=0.872),分析得出适宜SID Lys水平为0.592%。妊娠90d血清中胰岛素、类胰岛素样生长因子-I、孕酮、催乳素的含量都随着日粮SID Lys含量的增加呈现出先升高后下降的趋势。0.55%组和0.65%组的胰岛素含量显著高于0.75%组(0.01P0.05);0.55%组的类胰岛素样生长因子-I的含量显著高于0.75%组(0.01P0.05);孕酮含量0.45%组显著高于0.65%组和0.75%组(0.01P0.05);催乳素0.55%组显著高于0.35%组(0.01P0.05)。母猪总产仔数随着日粮SID Lys含量的增加呈现出先升高后下降的趋势,但未达到显著水平(P0.05)。仔猪初生重随着日粮中SID Lys含量的增加呈现出先升高后下降的趋势,且0.55%组显著高于其余各组(0.01p0.05)。仔猪28d血清中血清尿素氮的含量随着日粮sidlys水平的增加呈现出先下降后上升的趋势,0.45%组和0.55%组显著低于其余各组(0.01p0.05),且0.55%组含量最低。综合分析母猪妊娠90d血清尿素氮、胰岛素、仔猪哺乳期增重相关指标的二次曲线得出:本试验条件下,荣昌母猪妊娠1~90d日粮代谢能水平为11.75mj/kg时,sidlys水平为0.546%~0.592%具有最佳的繁殖性能。试验二:为研究荣昌母猪妊娠91~110d日粮适宜的标准回肠可消化赖氨酸适宜需要量,选取二胎次、体重相近、血缘相近的荣昌母猪55头按体重随机区分为5个处理,每个处理11头,饲喂sidlys水平分别为0.50%、0.60%、0.70%、0.80%、0.90%,代谢能为12.50mj/kg、粗蛋白为12.60%的日粮。配种至妊娠90d及哺乳期饲喂相同日粮。试验期从妊娠90d开始至仔猪断奶结束,测定荣昌母猪妊娠90d、妊娠110d、分娩时(分娩后24h内)、断奶时的体重及背膘厚、产仔数、产活仔数、仔猪初生重、仔猪14日龄体重、仔猪28日龄体重,母猪妊娠110d的血糖、血清尿素氮、血清总蛋白、胰岛素、类胰岛素样生长因子-i、催乳素以及妊娠110d母猪血清游离氨基酸。结果表明:母猪妊娠91~110d背膘厚变化随着日粮sidlys水平增加呈先出先升高后下降的趋势,0.70%组显著高于0.50%(0.01p0.05);妊娠110d母猪血清尿素氮及血糖含量随着日粮sidlys水平增加呈现出二次曲线变化。0.70%组血糖含量最高,显著高于其他各处理组(0.01p0.05);血清尿素氮0.80%组含量达到最低,且妊娠110d母猪血清血清尿素氮含量(y)与日粮sidlys水平(x)之间的关系为y=16.714x2-24.16x+10.496(r2=0.935),分析得出适宜sidlys水平为0.723%;妊娠110d胰岛素与类胰岛素样生长因子-i含量随着日粮sidlys含量的增加呈现出先升高后下降的趋势,且胰岛素0.75%组含量达到最大,显著高于0.50%组(0.01p0.05);妊娠110d母猪血清中游离赖氨酸含量0.85%组显著高于0.50%组(0.01p0.05),血清游离赖氨酸含量随日粮sidlys含量的增加呈现出先升高后下降的趋势;妊娠后期日粮sidlys水平对仔猪初生重有显著影响(0.01p0.05),仔猪初生重随着日粮中sidlys含量的增加呈上升趋势;哺乳仔猪1~14d体增重0.70%组显著高于0.80%和0.90%组。哺乳期仔猪14d、28d血清中尿素氮含量随着日粮中sidlys含量的增加呈先上升后下降趋势;母猪断奶至发情间隔虽没有达到显著水平(p0.05),但在数值上随着日粮sidlys水平的增加呈现出先下降后上升的趋势,0.80%组断奶至发情间隔天数最少。综合分析母猪妊娠期血清尿素氮、母猪妊娠后期体重变化、胰岛素、仔猪1~14d体增重相关指标的二次曲线分析得出:本试验条件下,当荣昌母猪妊娠91~110d日粮代谢能为12.50mj/kg时,sidlys水平为0.655%~0.723%具有最佳的繁殖性能。
[Abstract]:In this study, the sow pregnancy period was divided into two stages of pregnancy 1~90d and pregnancy 91~110d, and two experiments were carried out to study the requirement of the suitable standard ileum lysine for Rongchang sows during pregnancy. Test 1: To study the needs of the standard ileum for the standard ileum in the gestation of pregnant sows in Rongchang, and to select 50 Rongchang sows. 5 treatments were randomly divided into 5 treatments, each treated with 10 heads, and the levels of SID Lys were 0.35%, 0.45%, 0.55%, 0.65%, 0.75%, and the metabolic energy level was on the diet. The sows were fed from the pregnancy 91d to the end of the lactation period. In post 24h), the weight and back fat thickness of sows were measured during weaning, the number of births, the number of live births, the birth weight of piglets during childbirth were recorded, and the blood sugar, serum total protein, serum total protein, serum urea nitrogen, insulin, insulin like growth factor -I, progesterone, prolactin and lactating piglets, the blood sugar, total protein, urea nitrogen were measured. The 1~90d weight and back fat thickness of sow's gestation were increased first and then decreased with the increase of SID Lys in the diet, but did not reach a significant level (P0.05). The value of serum total protein, blood sugar, triglyceride and total cholesterol in the treatment groups of pregnancy 90d increased first and then decreased with the increase of SID Lys level in the diet. But there was no significant level (P0.05). The serum urea nitrogen content in each treatment group of pregnancy 90d decreased first and then increased with the increase of SID Lys level in the diet, and the 0.55% and 0.65% groups were significantly lower than 0.35% groups (0.01P0.05), the relationship between the serum urea nitrogen content of pregnancy 90d (Y) and the SID Lys level (X) of the diet was two times: Y=12 .571X2-14.889X+6.9424 (R2=0.872), it was found that the appropriate SID Lys level was insulin in 0.592%. pregnancy 90d serum, and the content of insulin like growth factor -I, progesterone and prolactin all increased and then decreased with the increase of SID Lys content in the diet, and the insulin content in the.0.55% and 0.65% groups was significantly higher than that of the 0.75% groups (0.01P0.05). The content of insulin like growth factor -I in the 0.55% groups was significantly higher than that in the 0.75% group (0.01P0.05), the progesterone content in 0.45% groups was significantly higher than that in the 0.65% and 0.75% groups (0.01P0.05), and the prolactin 0.55% group was significantly higher than the 0.35% group (0.01P0.05). The primary weight of the piglets increased with the increase of SID Lys content in the diet first and then decreased, and the 0.55% groups were significantly higher than the other groups (0.01p0.05). The content of serum urea nitrogen in the 28d serum of the piglets showed a tendency to descend first and then increase with the increase of sidlys level in the diet, and the content of the serum urea nitrogen in 28d serum of piglets showed a trend of descending and then rising, and the 0.45% and 0.55% groups were significantly lower than those in the 0.55% groups. The remaining groups (0.01p0.05), and the 0.55% groups were the lowest. A comprehensive analysis of the two curves of serum urea nitrogen, insulin and piglet feeding weight gain in sow pregnancy showed that, under the conditions of this test, the metabolic energy level of 1~90d diet in Rongchang sows was 11.75mj/kg, and sidlys was 0.546%~0.592% with the best reproductive performance. The test was two: In order to study the suitable standard of the standard ileum for the standard ileum of Rongchang sow pregnancy 91~110d, the 55 heads of Rongchang sows with similar body weight were randomly divided into 5 treatments, each treated with 11 heads, and the level of feeding sidlys was 0.50%, 0.60%, 0.70%, 0.80%, 0.90% respectively, and the metabolic energy was 12.50mj/kg, crude protein. For 12.60% of the diet, the same diet was fed to pregnancy 90d and lactation period. The test period from pregnancy 90d to weanling ended, the pregnancy 90d, pregnancy 110D, birth (24h after birth 24h), weaning weight and back fat thickness, litter size, birth weight, piglets' birth weight, piglet's 14 day weight, piglet's 28 day age weight, mother body weight, mother's 28 day weight, mother body weight, mother body weight, mother body weight, mother body weight, and mother weight of piglets 110D blood sugar, serum urea nitrogen, serum total protein, insulin, insulin like growth factor -i, prolactin and serum free amino acids in gestation 110D sows. The results showed that the change of 91~110d back fat thickness of sow pregnancy increased first and then decreased with the increase of sidlys level in the diet, which was significantly higher than 0.50% (0.01p0.05) in the 0.70% group. The serum urea nitrogen and blood sugar content in the serum of pregnant 110D sows showed two curves with the increase of sidlys level in the diet. The blood sugar content in group.0.70% was highest, which was significantly higher than that of other treatment groups (0.01p0.05); the content of serum urea nitrogen 0.80% groups reached the lowest level, and the serum serum albumin urea nitrogen content (y) and dietary sidlys level (x) in the serum of 110D sows of pregnancy were between. The relationship was y=16.714x2-24.16x+10.496 (r2=0.935), and the suitable level of sidlys was 0.723%. The content of 110D insulin and insulin-like growth factor -i in pregnancy increased first and then decreased with the increase of dietary sidlys content, and the content of insulin 0.75% groups reached the maximum, which was significantly higher than that of the 0.50% groups (0.01p0.05); pregnancy 110D sows. The content of free lysine in the 0.85% groups was significantly higher than that of the 0.50% groups (0.01p0.05). The content of free lysine in serum showed a tendency to increase first and then decrease with the increase of sidlys content in the diet. The level of sidlys in the diet of late pregnancy had a significant effect on the newborn weight of piglets (0.01p0.05), and the initial weight of the piglets increased with the increase of sidlys content in the diet. The 0.70% groups of 1~14d body weight gain of suckling piglets were significantly higher than that of 0.80% and 0.90% groups. The content of urea nitrogen in 14d and 28d serum of suckling piglets rose first and then decreased with the increase of sidlys content in the diet; the sow weaned to the estrus interval did not reach the significant level (P0.05), but in the numerical value increased with the increase of the level of sidlys in the diet. The number of weaning to estrus interval in the 0.80% groups was the least. The two curves of serum urea nitrogen, body weight change, insulin and 1~14d body weight gain of piglets during pregnancy were analyzed by two curves. The results were as follows: when the diet of Rongchang sows was metabolizable to 91~110d, sidlys The level of 0.655%~0.723% has the best reproductive performance.

【学位授予单位】:西南大学
【学位级别】:硕士
【学位授予年份】:2016
【分类号】:S828

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