牦牛PPARδ基因多态性与生长性状的相关性

发布时间：2018-05-01 18:57

本文选题：牦牛 + PPARδ基因　；参考：《甘肃农业大学学报》2017年03期

【摘要】：【目的】揭示牦牛PPARδ基因的遗传变异特征,发现与牦牛生长性状显著相关的候选分子标记,为牦牛分子育种积累基础资料.【方法】以96头牦牛血样为材料,运用DNA池测序和PCR-HRM等技术检测PPARδ基因的序列变异.【结果】检测到牦牛PPARδ基因内含子中2个SNPs.遗传变异特征分析显示,SNP1(g.10045A/G)和SNP2(g.10226A/G)位点属于中度多态性.连锁不平衡和单倍型分析表明,牦牛PPARδ基因2个多态位点之间为弱连锁,单倍型AA属于优势单倍型(频率为42.2%).方差分析表明,单个的SNP与牦牛生长性状有着显著或极显著的关联.【结论】牦牛PPARδ基因的2个SNPs与其生长性状显著相关,此结果可以应用于牦牛的分子育种实践.
[Abstract]:[objective] to reveal the genetic variation characteristics of yak PPAR 未 gene, and to find candidate molecular markers significantly related to yak growth traits, and to accumulate basic data for yak molecular breeding. [methods] 96 yak blood samples were used as materials. DNA pool sequencing and PCR-HRM were used to detect the sequence variation of PPAR 未 gene. [results] two SNPs in PPAR 未 gene intron of yak were detected. Genetic variation analysis showed that the SNP1 / 10045A / G) and SNP2 / 10226A / G) loci were moderately polymorphic. Linkage disequilibrium and haplotype analysis showed that the two polymorphic loci of yak PPAR 未 gene were weakly linked, and haplotype AA belonged to the dominant haplotype (the frequency was 42.2%). The analysis of variance showed that there was a significant or extremely significant correlation between single SNP and yak growth traits. [conclusion] two SNPs of yak PPAR 未 gene were significantly correlated with their growth traits, which could be applied to the practice of yak molecular breeding.
【作者单位】：甘肃农业大学生命科学学院;甘肃农业大学动物医学院;
【基金】：“十二五”农村领域国家科技计划项目“牦牛抗逆性状功能基因组学研究”(2013AA102505-3)
【分类号】：S823.85

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