吉林省内部分地区规模化养猪场疫苗免疫抗体水平检测与分析

发布时间：2018-05-01 19:25

本文选题：猪瘟 + 猪伪狂犬　；参考：《延边大学》2017年硕士论文

【摘要】：规模化猪场保持长期发展的重要前提就是保证疾病预防和控制,这关系到猪群的健康稳定。当前我国存在的严重危害猪场发展的三种主要传染病分别是猪瘟(CSF)、猪蓝耳病(PRRS)及猪伪狂犬病(PR)。健康猪若感染上述三种病毒性传染病,临床症状多会呈现高温、繁殖障碍等,猪场若大规模发病会给产生严重的经济损失。我国对于猪场疾病以预防为主、多采用注射疫苗等措施,那么疫苗的免疫效果就可通过抗体水平检测进行评价。为评估吉林省7个地区内规模化养猪场三种疾病的疫苗免疫情况,本研究应用哈尔滨兽医研究所研发酶联免疫分析试剂盒,采用ELISA方法对吉林省四平市等7个地区猪场送检的2 700份免疫后育肥猪血清进行猪瘟、猪繁殖与呼吸综合征(蓝耳病)及猪伪狂犬病的抗体免疫效果检测,根据检测结果分析吉林省地区猪场免疫水平。本试验通过ELISA方法对吉林省四平市等七个地区2 700份血清样本进行了猪瘟、猪蓝耳病及猪伪狂犬病的免疫抗体水平效果检测。结果表明,猪瘟抗体免疫水平的总阳性率为85.89%,其中四平市阳性率最高为89.56%,辽源市阳性率最高为99.11%,白山市阳性率最高为67.78%,长春市阳性率最高为75.33%,吉林市阳性率最高为90.67%,白城市阳性率最高为92.22%,通化市阳性率最高为84.00%。猪蓝耳病抗体水平的总阳性率为69.78%,其中四平市阳性率最高为75.33%,辽源市阳性率最高为88.44%,白山市阳性率最高为56.00%,长春市阳性率最高为50.67%,吉林市阳性率最高为91.67%,白城市阳性率最高为50.67%,通化市阳性率最高为80.00%。猪伪狂犬病抗体水平的总阳性率为66.00%,其中四平市阳性率最高为85.78%,辽源市阳性率最高为95.11%,白山市阳性率最高为52.00%,长春市阳性率最高为53.00%,吉林市阳性率最高为82.67%,白城市阳性率最高为34.00%,通化市阳性率最高为58.00%。结果表明,三种主要疫病中猪瘟疫苗的抗体免疫水平效果最好,猪蓝耳病疫苗的抗体免疫水平效果次之,猪伪狂犬病疫苗的抗体免疫水平效果较低。
[Abstract]:The important prerequisite for the long-term development of large-scale pig farms is to ensure disease prevention and control, which is related to the health and stability of pigs. At present, the three major infectious diseases that seriously endanger the development of pig farms in China are CSFV, PRRSs and PRPRs of pseudorabies. If the healthy pigs are infected with the above three viral infectious diseases, the clinical symptoms will be high temperature, reproductive disorders and so on. If the pig farm has a large-scale disease, it will cause serious economic losses. In our country, the prevention of pig farm diseases is the main method, and most of them are vaccinated, so the immune effect of the vaccine can be evaluated by antibody level test. In order to evaluate the vaccine immunity of three diseases in large scale pig farms in 7 regions of Jilin Province, the Elisa kit was developed by Harbin Veterinary Research Institute (Harbin Veterinary Research Institute). ELISA method was used to detect the antibody effect of swine fever, porcine reproductive and respiratory syndrome (RRS) and porcine pseudorabies (PRB) in 2 700 pig sera collected from pig farms in Siping City, Jilin Province. The immunological level of pig farms in Jilin Province was analyzed according to the results. In this study, 2 700 serum samples from Siping City, Jilin Province, were tested for antibody level of swine fever, blue ear disease and pseudorabies by ELISA method. The results show that The total positive rate of antibodies against swine fever was 85.89. The highest positive rate was 89.56 in Siping, 99.11in Liaoyuan, 67.78 in Baishan, 75.33 in Changchun, 90.67 in Jilin. The highest positive rate was 92.22 and the highest was 84.00 in Tonghua City. The total positive rate of antibody level of blue ear disease in pigs was 69.78. The highest positive rate was 75.33 in Siping, 88.44 in Liaoyuan, 56.00 in Baishan, 50.67 in Changchun, 91.67 in Jilin and Baicheng. The highest positive rate was 50.67 in Tonghua city and 80.005 in Tonghua city. The total positive rate of porcine pseudorabies antibody level was 66.00. The highest positive rate was 85.78 in Siping city, 95.11cm in Liaoyuan city, 52.00 in Baishan city, 53.00 in Changchun city, 82.67 in Jilin city, and 82.67 in Baicheng city. The highest positive rate was 34.00 in Tonghua and 58.00 in Tonghua. The results showed that the antibody immune level of swine fever vaccine was the best, the antibody level of swine blue ear disease vaccine was the second, and the antibody level of pig pseudorabies vaccine was lower than that of pig pseudorabies vaccine.
【学位授予单位】：延边大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S858.28

【相似文献】