免疫分子CD28及4-1BB在仔猪免疫疫苗前后表达变化的研究

发布时间：2018-05-02 21:01

本文选题：CD28 + 4-1BB　；参考：《河北科技师范学院》2015年硕士论文

【摘要】：猪瘟、蓝耳病是养猪生产中两个常发、重要的传染病,目前对于这两种传染病的防控主要以接种疫苗为主,但由于种种原因,免疫效果往往不尽人意。研究发现,CD28和4-1BB在动物机体免疫反应中起着至关重要的作用?CD28表达于CD4+、CD8+T细胞上,其配体为表达在APC上的B7,两者相互作用,为激活T细胞提供第二信号,如缺乏第二信号,T细胞进入无反应状态、甚至凋亡;4-1BB表达于活化T细胞和骨髓来源的单核细胞、多核细胞,对T细胞的数量起着双重作用。在CD28缺失或多次刺激而表达下降时,4-1BB信号能够独立的使T细胞活化,并且对T细胞的生存的延长具有重要的作用?在不同时期给仔猪免疫猪瘟、蓝耳病两种不同种类的疫苗,采用荧光定量方法,检测免疫前3天,免疫后3天、7天等不同种类疫苗仔猪外周血中CD28以及4-1BB的表达量的变化,结果显示:仔猪在接受猪瘟、蓝耳病两种不同种类的疫苗刺激后外周血中的淋巴细胞表达CD28在免疫后3天的表达量有所增加,与接种的疫苗的种类没有直接的关系;4-1BB在免疫7天以后的表达变化有所升高,可能是对维持T细胞的活化和生存起到作用?在免疫60天后结果显示:个体CD28和4-1BB之间的表达成反比?有可能是因为4-1BB对T细胞具有双重作用的原因,在无刺激情况下,4-1BB导致T细胞凋亡的作用?不同时期猪体内猪瘟、蓝耳病抗体效价的检测?28%仔猪个体存在猪瘟母源抗体时,在17日龄接种蓝耳病疫苗,25日龄接种猪瘟疫苗,用ELISA方法检测猪瘟和蓝耳病抗体效价。结果显示:免疫猪瘟疫苗3天后,24%个体为阳性,免疫7天、30天后,50%、83%个体为阳性;免疫蓝耳病疫苗3天后,6.7%个体为抗体阳性,7天后,16%个体为抗体阳性,14天后,86%个体为阳性,30天后100%个体为阳性。免疫30天后,猪瘟、蓝耳病两种比较,以蓝耳病疫苗免疫效果较好。对不同时期血液中IL-2的浓度检测结果表明:发现免疫前3天IL-2平均的表达浓度为383.8pg/mL,免疫后3天的IL-2平均表达浓度526.29pg/mL,免疫后7天IL-2的平均表达量为225.67pg/mL,免疫后14天IL-2的平均表达量为184.68pg/ml?接种疫苗后刺激猪血液中的IL-2表达量增加,但是与抗体的增长没有明显的关系?
[Abstract]:Swine fever and blue ear disease are two frequent and important infectious diseases in pig production. At present, the main prevention and control of the two diseases are mainly vaccinated, but because of various reasons, the immune effect is often unsatisfactory. It is found that CD28 and 4-1BB play a vital role in the immune response of animals. CD28 is expressed in CD4+, CD8+T cells On the other hand, the ligand is the expression of B7 on APC, which interact to provide second signals to activate T cells, such as the lack of second signals, T cells enter the non reactive state, or even apoptosis; 4-1BB is expressed in the activated T cells and bone marrow mononuclear cells, and the multinucleated cells play a double role in the number of T cells. When it falls, the 4-1BB signal can activate T cells independently and play an important role in prolonging the survival of T cells. In different periods, the piglets are immune to swine fever, two different kinds of blue ear diseases, using fluorescence quantitative method, 3 days before immunization, 3 days after immunization, 7 days, and 7 days in the peripheral blood of different kinds of piglets, and 4 The changes in the expression of -1BB showed that the expression of CD28 in the peripheral blood of piglets received swine fever and two different kinds of blue ear diseases increased after 3 days of immunization, and there was no direct relationship with the types of vaccinated vaccines; the expression of 4-1BB in the immune system increased after 7 days of immunization. To maintain the activation and survival of T cells? After 60 days of immunization, the results showed that the expression of CD28 and 4-1BB was inversely proportional to the effect of 4-1BB on T cells, the effect of 4-1BB on the apoptosis of T cells in the absence of stimulation? The detection of the antibody titer of the swine fever and the blue ear disease in the non simultaneous pigs? 28% The pigs were inoculated with blue ear disease vaccine at 17 days of age and inoculated with swine fever vaccine at the age of 17 days. The antibody titers of swine fever and blue ear disease were detected at 25 days of age. The results showed that 24% individuals were positive for 3 days, 7 days, 30 days, 50% and 83% were positive, and 6.7% individuals were immunized after 3 days of immune blue ear disease vaccine. 6.7% individuals were antibodies. Positive, 7 days, 16% of the antibody positive, 14 days, 86% of the positive, 30 days after 100% of the positive. 30 days after the immune 30, the swine fever, blue ear disease two comparison, blue ear vaccine immune effect is better. The concentration of IL-2 in the blood of different periods of time test showed that the average expression of IL-2 was 383.8pg/mL, immunization for 3 days before the onset of immunization. The average expression concentration of IL-2 was 526.29pg/mL at the last 3 days. The average expression of IL-2 was 225.67pg/mL after 7 days after immunization. The average expression of IL-2 in 14 days after immunization was 184.68pg/ml? After vaccination, the IL-2 expression in the blood of pigs was increased, but there was no significant relationship with the increase of antibody.

【学位授予单位】：河北科技师范学院
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S858.28

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