藏鸡源食窦魏斯氏菌的抑菌特性研究

发布时间：2018-05-03 21:17

本文选题：食窦魏斯氏菌 + 细菌素　；参考：《畜牧与兽医》2017年12期

【摘要】：为了解藏鸡源益生菌的抑菌特性,选用10只体质健康的1月龄藏鸡作为益生菌的分离来源。以肠炎沙门菌(Salmonella enterica)为指示病原菌,通过琼脂扩散试验对分离菌株进行抑菌特性判定,共筛选到6株具有抑制肠炎沙门菌生长繁殖的菌株,抑菌圈直径均在8~28 mm之间,其中从小肠黏膜中分离到的食窦魏斯氏菌(Weissella cibaria)的抑菌圈直径高达276 mm,明显高于其他菌株和常用抗生素。将该菌株命名为WT1,并对WT1的抑菌特性和生物学活性进行深入研究。结果显示:WT1所产细菌素作用肠炎沙门菌8 h后,肠炎沙门菌大量死亡;基因序列分析显示,WT1益生基因entA;WT1具有良好的热稳定性和耐酸性。研究表明,藏鸡源食窦魏斯氏菌具有良好的抑菌特性和生物学活性,可以用于家禽肠炎沙门菌感染的治疗,在一定程度上缓解抗生素的负面影响。
[Abstract]:In order to understand the bacteriostatic characteristics of probiotics from Tibetan chickens, 10 healthy 1-month-old Tibetan chickens were selected as the source of isolation of probiotics. Using Salmonella entericia as the indicator pathogen, the bacteriostasis characteristics of the isolated strains were determined by Agar diffusion test. A total of 6 strains were screened to inhibit the growth and reproduction of Salmonella enterica, and the diameter of the inhibition circle was between 8mm and 28mm. The bacteriostasis diameter of Weissella cibaria isolated from intestinal mucosa was 276mm, which was significantly higher than that of other strains and antibiotics. The strain was named WT1, and the bacteriostasis and biological activity of WT1 were studied. The results showed that a large number of Salmonella enteritidis died after 8 h treatment with the bacteriocin produced by WWT1, and the gene sequence analysis showed that entAgnt WT1 probiotic gene had good thermal stability and acid tolerance. The results showed that Weiss's bacilli from Tibetan chicken had good bacteriostasis and biological activity and could be used in the treatment of Salmonella enteritis infection in poultry and alleviate the negative effects of antibiotics to some extent.
【作者单位】：华中农业大学动物医学院;西藏农牧学院动物科学学院;
【基金】：西藏自治区科技厅重点项目(2012BAD3B03) 国家肉牛牦牛产业技术体系(CARS-38)
【分类号】：S852.6

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