基因3型猪戊型肝炎病毒浙江株全基因组序列分析（英文）

发布时间：2018-05-05 04:15

本文选题：猪戊型肝炎病毒 + 全基因组序列　；参考：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2017年06期

【摘要】：目的:扩增浙江地区基因3型戊型肝炎病毒全基因组序列,分析其分子特征及与国内外3型戊型肝炎病毒进行比较。创新点:首次分析报道浙江地区基因3型猪戊型肝炎病毒全序列。方法:利用套式PCR和末端快速扩增法对分离自浙江地区的戊型肝炎病毒进行分段扩增,经克隆测序后拼接全基因组。进一步利用生物信息学方法对基因组结构、开放阅读框及其基因型等进行分析,并与国内外戊型肝炎毒株进行比较分析。结论:浙江株戊型肝炎病毒(Zh J-PJ050-3)基因组由7223个核苷酸组成,其中3'非编码区含有23个碱基的poly(A)尾。全基因组包含ORF1、ORF2和ORF3三个主要基因,长度分别为5109、1983和342 bp。进化分析显示,ZhJ-PJ050-3为基因3型,与国内唯一报道的上海株3型毒株以及多数日本毒株共属3b亚型。与国内外戊型肝炎序列比对发现,ZhJ-PJ050-3基因组共有24个特有的点突变,且在ORF1中含有一个亮氨酸缺失。这些分子特征将为下一步的研究提供方向。
[Abstract]:Objective: to amplify the whole genome sequence of hepatitis E virus type 3 in Zhejiang province and analyze its molecular characteristics and compare it with that of hepatitis E virus type 3 at home and abroad. Innovation: the whole sequence of porcine hepatitis E virus type 3 in Zhejiang province was analyzed for the first time. Methods: hepatitis E virus isolated from Zhejiang province was amplified by nested PCR and terminal rapid amplification. The whole genome was cloned and sequenced. The genome structure, open reading frame and its genotypes were further analyzed by bioinformatics, and compared with those of hepatitis E virus strains at home and abroad. Conclusion: the Zh J-PJ050-3 genome of hepatitis E virus from Zhejiang province consists of 7223 nucleotides, of which the 3'noncoding region contains 23 base polymorphic DNA tails. The whole genome contains three major genes, ORF1 ORF2 and ORF3, with lengths of 5109 and 342bprespectively. Phylogenetic analysis showed that ZhJ-PJ050-3 was a gene type 3, which belonged to subtype 3b with the Shanghai strain 3 and most Japanese strains reported in China. Comparing with the sequence of hepatitis E at home and abroad, it was found that there were 24 unique point mutations in the ZhJ-PJ050-3 genome, and there was a leucine deletion in ORF1. These molecular characteristics will provide a direction for further research.
【作者单位】： Zhejiang
【基金】：supported by the Key Research and Development Program of Zhejiang Province(No.2015C02044) the National Key Technology R&D Program of China(No.2016YFD0501100) the National Natural Science Foundation of China(No.31301492) the Zhejiang Entry-Exit Inspection and Quarantine Bureau(No.2016-ZKZ-02),China
【分类号】：S852.65

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