益阳市犬弓首蛔虫感染情况调查及ITS、cox1基因的多态性分析

发布时间：2018-05-05 21:03

本文选题：犬弓首蛔虫 + 湖南省益阳市　；参考：《湖南农业大学》2015年硕士论文

【摘要】：犬弓首蛔虫病是由犬弓首蛔虫(Toxocara canis)寄生于犬和其它动物(包括人)而引起的一种动物源性寄生虫病。目前湖南省益阳市地区还没有相关的研究报导。本文通过在2012年至2014年对湖南省益阳市的犬弓首蛔虫的感染情况进行调查,并运用分子生物学方法对其核糖体内部转录间隔区(ITS rDNA)和线粒体细胞色素氧化酶I基因(coxl)进行多态性研究,得出结果如下：1.应用饱和盐水漂浮法检查犬粪便样品417份,其中229份检测出犬弓首蛔虫虫卵,平均感染率为54.9%。2.驱虫试验结果显示,芬苯达唑、伊维菌素、左旋咪唑三种药物均能有效驱除犬弓首蛔虫,其中芬苯达唑效果最佳。3.应用分子生物学方法对犬弓首蛔虫的核糖体内部转录间隔区(Internal transcrib ed spacer ribosome DNA -ITS rDNA)和犬弓首蛔虫的线粒体细胞色素氧化酶I基因(cytochrome c oxidase subunit 1-cox1)进行多态性研究,结果显示：11株犬弓首蛔虫均得到472 bp的ITS-1 rDNA序列,且种内相似率为57.6%-76.9%,种间相似率为57.6%-76.9%；11株犬弓首蛔虫均得到323 bp的ITS-2 rDNA序列,序列种内相似率达99.4%以上,种间相似率均在46.7%-77.4%之间；线粒体coxl基因的种内相似性为99.3%-99.8%,种间相似性为85.8%-88.8%。
[Abstract]:Ascaris canis is an animal parasitic disease caused by Toxocara canis parasitic on dogs and other animals (including human beings). At present, there is no related research report in Yiyang area of Hunan Province. From 2012 to 2014, we investigated the infection of Ascaris canis in Yiyang City, Hunan Province. The polymorphism of its rDNA and mitochondrial cytochrome oxidase I gene was studied by molecular biology. The results were as follows: 1. The fecal samples of 417 dog feces were examined by saturated brine floating method, 229 of which were found to be eggs of Ascaris canis, and the average infection rate was 54.9. 2. The results of insect repellent test showed that fenbendazole, ivermectin and levamisole could effectively eliminate Ascaris canis, among which fenbendazole was the best. The polymorphism of internal transcrib ed spacer ribosome DNA-its rDNA in internal ribosomal spacer of Ascaris canis and cytochrome c oxidase subunit 1-cox1 gene of Ascaris canis was studied by molecular biology. The results showed that the ITS-1 rDNA sequence of 472bp was obtained in all 11 strains of Ascaris canis, and the intraspecific similarity rate was 57.6- 76.9. the interspecific similarity rate was 57.6- 76.9bp, and the ITS-2 rDNA sequence was 323bp for 11 strains of Ascaris canis. The similarity rate was 99.4% and 46.7- 77.4% respectively. The intraspecific similarity of mitochondrial coxl gene was 99.3-99.8, and the interspecific similarity was 85.8% -88.8%.
【学位授予单位】：湖南农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S858.292

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