犬肿瘤诊断、治疗及环氧合酶-2的表达

发布时间：2018-05-07 15:10

本文选题：犬肿瘤 + 流行病调查　；参考：《南京农业大学》2015年博士论文

【摘要】：随着我国犬的养殖数量日渐增多,随之而来的犬病发生率也在上升,犬的肿瘤疾病就是其中之一,且有日渐增多的趋势,但尚未引起宠物饲养者的重视。为探讨犬肿瘤临床发病规律,在江浙沪地区部分宠物医院门诊收集了133例犬肿瘤自然发生病例,对其临床症状、发病部位、品种、年龄、性别、饮食情况和转移情况等进行调查;用外科手术的方法摘除56例临床自然发生的犬肿瘤,将病料固定、切片、染色后,通过显微镜观察组织结构和细胞形态,并进行肿瘤分类和病变类型;探讨环氧合酶-2(COX-2)与犬肿瘤的发生、发展的关系,本研究收集术后的肿瘤组织46份和在浙江大学动物医院解剖的犬正常乳腺、皮肤、睾丸、肛周、子宫组织各6份,将上述组织包埋的蜡块做成切片,应用免疫组织化学S-P染色法检测COX-2在46份犬肿瘤组织及30份正常组织中的表达情况。本试验得到以下结果:1调查门诊收集的133例临床自然发生的犬肿瘤病例,结果显示:肿瘤发病早期一般不表现全身症状,恶性肿瘤发生转移时会有恶病质的表现;乳腺和皮肤及软组织是犬临床高发的肿瘤部位,分别占全部患犬的42.11%和24.81%;京巴犬、杂交犬、贵宾犬、博美犬和西施犬是犬肿瘤高发的品种,分别占全部患犬的19.55%、14.29%、11.28%、8.27%和7.52%;患病犬年龄从1-15岁大小不等,平均年龄为9.39岁,6-10岁年龄段的患犬发病率最高;一些肿瘤类型与性别、是否绝育有关;日常饮食与犬肿瘤的发生有关,肉拌饭类、纯肉加火腿肠类、狗粮加肉类和纯狗粮类,发病比例分别为39.85%、26.32%、21.05%和12.78%,其中纯狗粮饲养的犬发病率明显较低。2用外科手术的方法摘除56例临床自然发生的犬肿瘤,结果显示:外科手术是犬肿瘤最常用的治疗方法,56例犬肿瘤中30例为恶性肿瘤,26例为良性肿瘤。根据肿瘤不同组织类型分类,29例乳腺肿瘤中恶性肿瘤21例,包括复合癌、导管乳头状癌、实质型癌、未分化癌、黏液癌、纤维肉瘤、骨肉瘤和癌肉瘤;良性肿瘤8例,包括单纯型腺瘤、复合型腺瘤、纤维腺瘤和乳腺增生。12例皮肤及软组织肿瘤中恶性肿瘤3例,包括皮脂腺癌和脂肪肉瘤;良性肿瘤9例,包括基底细胞瘤、纤维瘤、血管周细胞瘤、皮脂腺瘤、皮脂腺上皮瘤和毛母细胞瘤。5例肛周肿瘤中恶性肿瘤3例为肛周腺癌;良性肿瘤2例为肛周腺瘤;6例阴道和子宫肿瘤中恶性肿瘤3例,包括纤维肉瘤和平滑肌肉瘤;良性肿瘤3例为平滑肌瘤。4例睾丸肿瘤都是良性肿瘤,包括支持细胞瘤和间质细胞瘤。3 COX-2在犬肿瘤中的阳性表达主要位于肿瘤细胞的胞浆内及胞核周围,为棕黄色颗粒。46例犬肿瘤中30例COX-2呈强阳性表达,5例呈阳性表达,阳性表达率为76.1%(35/46);30份正常组织中COX-2的阳性表达率为20%(6/30)。COX-2在犬肿瘤和正常组织上的表达差异有统计学意义(P0.05)。46例进行免疫组化染色的肿瘤中,19例良性肿瘤中15例COX-2呈强阳性表达,阳性表达率为78.95%;27例恶性肿瘤中15例COX-2呈强阳性表达,5例呈阳性表达,阳性表达率为74.1%。COX-2在犬良、恶性肿瘤上的表达无统计学意义(P0.05)。提示COX-2与犬肿瘤的发生和发展有关系。
[Abstract]:With the increasing number of dog breeding in China, the incidence of canine disease is increasing, and the dog's tumor disease is one of them, and there is a growing trend, but it has not attracted the attention of the pet owners. In order to explore the clinical law of the dog's tumor, 133 cases of dog tumor were collected from the outpatient of the pet hospital in Jiangsu, Zhejiang and Shanghai. In case of cases, the clinical symptoms, location, variety, age, sex, diet and metastasis were investigated, and 56 cases of canine tumors that were naturally occurring were removed by surgical procedure, and the tissue structure and cell morphology were observed by microscope, and the tumor classification and pathological type were observed by microscope. To investigate the relationship between cyclooxygenase -2 (COX-2) and the occurrence and development of canine tumor. This study collected 46 tumor tissues after operation and 6 tissues of normal breast, skin, testis, perianal and uterus dissected at the animal hospital of Zhejiang University. The wax blocks embedded in the above tissue were cut into slices, and COX-2 was detected by immunohistochemical S-P staining method in 46. The following results were obtained in this test: 1 the results of 133 cases of naturally occurring canine tumor cases collected in the outpatient clinic were investigated. The results showed that the early stage of the tumor usually did not show systemic symptoms, and the malignant tumor appeared when the malignant tumor was transferred; the breast and skin and soft tissue were the dog's face. The high incidence of tumours in the bed accounted for 42.11% and 24.81% of the total dogs; Beijing, hybrid, poodle, BBO and Xishi were the high incidence of canine tumors, which accounted for 19.55%, 14.29%, 11.28%, 8.27% and 7.52% of all dogs. The age of the sick dog ranged from 1-15 years to 9.39 and 6-10 years old. The incidence of some tumor types was related to sex and sterilization; daily diet was associated with the occurrence of canine tumors, meat mixing, pure meat and ham sausage, dog food plus meat and pure dog food, respectively, 39.85%, 26.32%, 21.05% and 12.78%, respectively. The incidence of pure dog food in dogs was significantly lower than that of 56 cases of.2 surgery. The results showed that surgery was the most common treatment for canine tumors, 30 of 56 cases of canine tumors were malignant and 26 were benign. According to the classification of different tissue types, 21 cases of malignant tumor in 29 breast tumors, including compound carcinoma, ductal papillary carcinoma, parenchyma carcinoma, undifferentiated carcinoma, mucus cancer, fiber 8 cases of benign tumors, including simple adenoma, compound adenoma, fibroadenoma, and mammary gland hyperplasia in 3 cases of.12 and soft tissue tumors, including sebaceous adenocarcinoma and liposarcoma, and 9 cases of benign tumor, including basal cytoma, fibroma, pericytoma, sebaceous adenoma, and sebaceous epithelioma. And 3 cases of malignant tumor of perianal tumor in.5 cases, 2 cases of benign tumor were perianal adenoma, 6 cases of malignant tumor in vaginal and uterine tumor, 3 cases of malignant tumor, including fibrosarcoma and smooth muscle sarcoma, 3 cases of benign tumor and.4 case of leiomyoma, benign tumor, including support cell tumor and interstitial cell tumor.3 COX-2 The positive expression in the canine tumor was mainly located in the cytoplasm and the nucleus of the tumor cells. 30 cases of COX-2 were strongly positive in.46 cases of brown yellow granules. The positive expression was 76.1% (35/46), and the positive expression rate of COX-2 in 30 normal tissues was 20% (6/30).COX-2 in canine tumor and normal tissue. The difference was statistically significant (P0.05) in the tumor of.46 immunohistochemical staining, 15 cases of benign tumors were strongly positive in 19 cases of benign tumor, and the positive expression rate was 78.95%. 15 cases of COX-2 were strongly positive in 27 cases of malignant tumor and 5 cases were positive, the positive expression rate was 74.1%.COX-2 in canine, and there was no statistical significance in the malignant tumor. P0.05), suggesting that COX-2 is related to the occurrence and development of tumor in dogs.

【学位授予单位】：南京农业大学
【学位级别】：博士
【学位授予年份】：2015
【分类号】：S858.292

【参考文献】