免疫组化检测伪狂犬病毒感染大鼠后在其组织中分布规律的研究

发布时间：2018-05-08 16:01

本文选题：伪狂犬病病毒 + 大鼠模型　；参考：《中国预防兽医学报》2017年05期

【摘要】：伪狂犬病毒(PRV)能够感染神经元细胞并且跨突触传播,该特性使它成为了一种新兴的神经示踪病毒。为探究PRV在动物体内分布情况,本研究构建了PRV人工感染大鼠模型,通过免疫组化技术检测了PRV在大鼠体内各组织器官中的增殖分布情况,同时还将免疫组化检测结果与PCR检测结果相比较。结果表明利用免疫组化技术分别在PRV感染72 h、78 h、84 h、96 h、104 h、108 h后开始依次在大鼠的脑、肺、肾、脾、肝、心检测到PRV。同时在总计36个PRV检测样品中,PCR检测的检出率为50%,免疫组化检测的检出率为36%,两者的符合率为72%。本研究利用免疫组化技术观察PRV感染大鼠后在体内增殖分布规律和病变程度,为进一步探索RV的组织嗜性以及机体抗病毒作用机制奠定基础。
[Abstract]:Pseudorabies virus (PRV) can infect neuron cells and spread across synapses, which makes it a new neurotracer virus. In order to investigate the distribution of PRV in animals, a rat model of PRV infection was established, and the proliferation and distribution of PRV in various tissues and organs of rats were detected by immunohistochemical technique. At the same time, the results of immunohistochemistry and PCR were compared. The results showed that PRV was detected in the brain, lung, kidney, spleen, liver and heart of the rats at 72 h, 78 h, 84 h, 96 h and 104 h and 108 h after infection by immunohistochemical technique. At the same time, the detection rate of PRV and immunohistochemistry were 50 and 36, respectively. The coincidence rate of the two methods was 72%. In this study, immunohistochemical technique was used to observe the distribution of proliferation and pathological changes after PRV infection in rats, which laid a foundation for further exploring the tissue eosinophilia of RV and the mechanism of antiviral action of RV.
【作者单位】：四川农业大学动物医学院;四川农业大学动物疫病与人类健康四川省重点实验室;
【基金】：四川省科技支撑计划(2014NZ0043、2017NZ0038) 国家“十二五”科技支撑计划项目(2015BAD12B04) 长江学者与创新团队发展计划(IRT13083)
【分类号】：S852.65

【相似文献】