直接法与替代法测定羊草对肉用绵羊代谢能的比较研究

发布时间：2018-05-09 19:37

本文选题：直接法 + 替代法　；参考：《动物营养学报》2016年02期

【摘要】：本试验旨在比较直接法和替代法测定羊草对肉用绵羊代谢能。选用体重约为45.00kg的体况良好的杂交肉用羯羊30只,采用随机区组设计,分为5个处理,每个处理6个重复,每个重复1只羊,分别饲喂采用直接法的全羊草饲粮、用于替代法的基础饲粮(精粗比为1∶1)和3个试验饲粮,3个试验饲粮分别以60%、40%、20%羊草替代基础饲粮。饲粮均于08:00、18:00各饲喂600 g。试验每期19 d,包括预试期10 d,正试期9 d。结果表明:干物质、有机物、总能、粗蛋白质表观消化率为基础饲粮组20%组40%组60%组全羊草饲粮组,组间差异显著(P0.05)。消化能与代谢能为基础饲粮组20%组40%组60%组全羊草饲粮组,组间差异显著(P0.05)。代谢能与消化能比各组饲粮差异不显著(P0.05)。直接法和替代法对测定原料羊草的粗蛋白质、中性洗涤纤维表观消化率影响不显著(P0.05)。直接法中全羊草饲粮组的羊草干物质、有机物、总能表观消化率显著高于60%组(P0.05),而与40%组、20%组之间差异不显著(P0.05)。全羊草饲粮组羊草的消化能和代谢能显著高于60%组(P0.05),与40%组、20%组之间差异不显著(P0.05)。综合得出,直接法与替代法在测定羊草的代谢能时,替代比例不同会对结果产生显著影响,在采用替代法测定羊草的代谢能时,最佳的替代比例为20%。
[Abstract]:The purpose of this study was to compare direct and substitute methods for the determination of metabolic energy of Leymus chinensis for meat sheep. Thirty crossbred sheep with good body weight (45.00kg) were selected and randomly divided into 5 treatments with 6 replicates and 1 sheep each, fed with a direct diet of whole Leymus chinensis. The basal diet (1: 1) and three experimental diets were replaced by 60% Leymus chinensis with 60% Rhizoma Leymus chinensis, respectively. The diets were fed at 8: 00: 00, 18: 00, and each was fed at 600 g. Each trial period was 19 days, including 10 days of pretest and 9 days of trial. The results showed that dry matter, organic matter, total energy and apparent digestibility of crude protein were the basic diet of 20% diet group, 40% group and 60% whole sheep grass diet group, and the difference between groups was significant (P 0.05). Digestible energy and metabolic energy were the basic diet of 20% group, 40% group, 60% group, whole sheep grass diet group, the difference between groups was significant (P 0.05). There was no significant difference in metabolic energy and digestible energy between the two groups (P 0.05). The direct method and substitution method had no significant effect on the apparent digestibility of crude protein and neutral detergent fiber of Leymus chinensis. The total apparent digestibility of dry matter and organic matter in the whole Leymus chinensis diet group was significantly higher than that in the 60% group, but there was no significant difference between the 40% group and the 40% group (20%). The digestibility and metabolic energy of the whole Leymus chinensis diet group was significantly higher than that of the 60% group (P0.05N), but there was no significant difference between the diet group and the 40% group (P0.05%). It is concluded that the difference of substitution ratio between direct method and substitution method in determining metabolic energy of Leymus chinensis has a significant effect on the results. The best substitution ratio is 20% when the substitution method is used to determine the metabolic energy of Leymus chinensis.
【作者单位】：新疆农业大学动物科学学院;中国农业科学院饲料研究所农业部饲料生物技术重点实验室;金陵科技学院动物科学与技术学院;
【基金】：秸秆饲料生物转化技术研究与示范(20120304202) 国家肉羊产业技术体系(CARS-39)
【分类号】：S826.5

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