猪源肺炎克雷伯氏菌分离菌株耐药性调查及防控技术研究

发布时间：2018-05-11 06:00

本文选题：肺炎克雷伯氏菌 + 猪　；参考：《南京农业大学》2015年硕士论文

【摘要】：肺炎克雷伯氏菌是一种很容产生耐药性的革兰氏阴性杆菌,泛耐药性克雷伯氏菌是"超级细菌"的一种,人医上对肺炎克雷伯氏菌的耐药性研究较多,而兽医上对该细菌还没有足够重视,没有人对肺炎克雷伯氏菌进行过系统的调研。本研究采用传统的生化试验方法对新乡市2012年～2013年间猪源肺炎克雷伯氏菌进行了分离鉴定,并用PCR方法进行了验证。通过药敏试验纸片方法(K-B法)对分离的肺炎克雷伯氏菌进行了耐药性分析,并总结了综合防控猪源肺炎克雷伯氏菌病的实施措施,现将主要研究结果简述如下:1.猪源肺炎克雷伯氏菌分离鉴定采集临床病料进行增菌,然后采用鉴别培养基DHL琼脂和MIAC琼脂对克雷伯氏菌进行初步分离,反复纯化后对细菌进行生化鉴定,最终从82份样品中,鉴定为克雷伯氏菌属的有26株,克雷伯氏菌分离率为31.71%,其中产酸克雷伯氏菌4株,占克雷伯氏菌总数的15.4%;肺炎克雷伯氏菌22株,各亚种数量分别为:肺炎克雷伯氏菌19株,占克雷伯氏菌73.1%;臭鼻克雷伯氏菌2株,占克雷伯氏菌总数的7.7%;鼻硬结克雷伯氏菌1株,占克雷伯氏菌总数的3.8%,未确定种的克雷伯氏菌0株。与卓超等人2009年、2010年报道的关于人感染肺炎克雷伯氏菌的数据比较吻合。2.猪源肺炎克雷伯氏菌耐药性研究为了解新乡市范围内猪源肺炎克雷伯氏菌的耐药情况,采用纸片法(K-B法)对临床分离的菌株进行药敏试验,并对产ESBLs酶和产AmpC酶菌株进行监测。结果临床分离的猪源肺炎克雷伯氏菌22株中,产ESBLs酶的有7株,产AmpC酶的有1株,不产生ESBLs和AmpC酶的14株。未发现耐碳青霉烯类菌株。分离的所有肺炎克雷伯氏菌对一代头孢、磺胺类药物和四环素耐药性较强。其中不产酶菌株对除一代头孢、磺胺类药物和四环素类以外的抗生素均比较敏感,产酶菌株对亚胺培南、氨曲南、多粘菌素B头孢吡肟以及头孢联合酶抑制剂的药物还有效。与人医相的结果比较吻合。3.猪源肺炎克雷伯氏菌综合防控研究通过克雷伯氏菌灭活疫苗试验证明了免疫可以很好的控制猪肺炎克雷伯氏菌病的流行,通过比较,经验治疗和药敏试验筛选药物治疗的结果,证明药敏试验在临床应用的重要性。最后总结归纳出了防控猪肺炎克雷伯氏菌病的综合措施。以上研究为防控猪肺炎克雷伯氏菌病提供了数据资料和技术指导。4.结论研究表明,肺炎克雷伯菌在猪体内确实存在。产ESBLs酶和AmpC酶肺炎克雷伯菌已证实可以在猪体内分离到。本研究对于预防和控制猪肺炎克雷伯菌病进行了一些初步的调查和研究,为兽医工作者防控该病提供一些基础数据。
[Abstract]:Klebsiella pneumoniae is a gram-negative bacilli that can produce drug resistance. Pan-resistant Klebsiella pneumoniae is one of the "super bacteria." there is much research on the resistance of Klebsiella pneumoniae to human doctors. Veterinarians have not paid enough attention to the bacteria, and no systematic investigation of Klebsiella pneumoniae has been conducted. In this study, the traditional biochemical test method was used to isolate and identify Klebsiella pneumoniae from the year of 2012 to 2013 in Xinxiang City, and PCR method was used to verify the isolation and identification of Klebsiella pneumoniae. Antimicrobial resistance of isolated Klebsiella pneumoniae was analyzed by using the method of drug susceptibility test (K-B), and the measures to prevent and control Klebsiella pneumoniae from swine were summarized. The main results are summarized as follows: 1. Isolation, identification and identification of Klebsiella pneumoniae isolated from swine, clinical materials were collected to increase the bacteria, and then DHL Agar and MIAC Agar were used to isolate Klebsiella pneumoniae. After repeated purification, the bacteria were biochemically identified. Finally, 82 samples were collected. Of the 26 strains identified as Klebsiella, the isolation rate of Klebsiella was 31.71, of which 4 were Klebsiella, accounting for 15.410% of the total number of Klebsiella pneumoniae, 22 strains of Klebsiella pneumoniae, 19 strains of Klebsiella pneumoniae. Accounted for 73.1% of Klebsiella, 2 strains of Klebsiella odorifolia, 7. 7% of the total number of Klebsiella, 1 strain of Klebsiella sinensis, 3. 8% of the total number of Klebsiella, 0 strains of Klebsiella spp. And Zhuo Chao et al. 2009, 2010 reported about human infection of Klebsiella pneumoniae data is consistent with. 2. 2. Study on Drug Resistance of Klebsiella pneumoniae isolated from Swine in order to understand the drug resistance of Klebsiella pneumoniae in Xinxiang City, the susceptibility test of clinically isolated strains was carried out by using K-B method and ESBLs producing and AmpC producing strains were monitored. Results among 22 strains of Klebsiella pneumoniae isolated clinically, 7 strains produced ESBLs enzyme, 1 strain produced AmpC enzyme, and 14 strains did not produce ESBLs and AmpC enzymes. No carbapenem resistant strains were found. All isolates of Klebsiella pneumoniae were resistant to primary cephalosporins, sulfonamides and tetracycline. The non-enzyme producing strains were sensitive to antibiotics except for the first generation of cephalosporins, sulfanilamides and tetracyclines. The enzyme producing strains were also effective against imipenem, aztreonam, polymyxin B cefepime and ceftaxime. The results were in good agreement with the results of human medicine. A comprehensive study on the prevention and control of Klebsiella pneumoniae from swine the inactivated vaccine test of Klebsiella pneumoniae proved that immunization can control the epidemic of Klebsiella pneumoniae very well. The results of drug treatment were screened by comparison, experience treatment and drug sensitivity test. To prove the importance of drug sensitivity test in clinical application. Finally, the comprehensive measures of prevention and control of Klebsiella pneumoniae were summarized. These studies provide data and technical guidance for the prevention and control of Klebsiella pneumoniae. Conclusion the study shows that Klebsiella pneumoniae does exist in pigs. Klebsiella pneumoniae producing ESBLs and AmpC enzymes have been confirmed to be isolated in pigs. In this study, the prevention and control of Klebsiella pneumoniae were investigated and studied, which provided some basic data for the prevention and control of Klebsiella pneumoniae.
【学位授予单位】：南京农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S852.61

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