一株貉子源毛滴虫的分离鉴定

发布时间：2018-05-11 19:20

本文选题：貉子 + 毛滴虫　；参考：《中国预防兽医学报》2017年08期

【摘要】：为进一步鉴定从河北秦皇岛地区腹泻貉子的盲肠中新分离得到的一株毛滴虫,本研究分别对其进行了分离与体外培养、形态学观察及18S rRNA和ITS1-5.8S rRNA-ITS2基因序列相似性分析。形态学观察结果表明从貉子体内分离的毛滴虫具有4根前鞭毛和一根独立鞭毛,为人五毛滴虫形态结构。18S rRNA和ITS1-5.8SrRNA-ITS2基因序列与GenBank中登录的人五毛滴虫基因序列的相似性均为99%。基于18S rRNA和ITS1-5.8SrRNA-ITS2两种基因构建系统进化树,结果显示本研究从貉体内分离的毛滴虫与人五毛滴虫处于同一进化分支,亲缘关系较近。综合以上鉴定结果表明本研究从秦皇岛地区貉子体内所分离的这株毛滴虫为人五毛滴虫。由此推断貉子毛滴虫病可能由人体内的毛滴虫感染所致。
[Abstract]:In order to further identify a Trichomonas trichomonas newly isolated from the cecum of raccoon in Qinhuangdao area, Hebei Province, the Trichomonas trichomonas was isolated and cultured in vitro, morphological observation and sequence similarity analysis of 18s rRNA and ITS1-5.8S rRNA-ITS2 genes were carried out. Morphological observation showed that trichomonas had four proflagellum and one independent flagellum. The similarity between the morphological structure of Trichomonas hominis. 18s rRNA and ITS1-5.8SrRNA-ITS2 gene sequence and that of human trichomonas hominis gene recorded in GenBank was 99g. The phylogenetic tree was constructed based on 18s rRNA and ITS1-5.8SrRNA-ITS2 genes. The results showed that Trichomonas trichomonas isolated from raccoon dog and human trichomonas were in the same evolutionary branch and had close phylogenetic relationship. The results showed that the Trichomonas trichomonas was isolated from the raccoon in Qinhuangdao area. It is concluded that trichomonas trichomoniasis may be caused by trichomonas infection in humans.
【作者单位】：河北科技师范学院动物科技学院/河北省预防兽医重点实验室;吉林大学畜牧兽医学院;
【基金】：河北省高校科学技术研究优秀青年基金项目(YQ2014012) 河北省青年拔尖人才项目
【分类号】：S858.92

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