利用纤维素降解菌固态发酵提高粗饲料营养价值的研究

发布时间：2018-05-12 03:36

  本文选题：微生物发酵 + 粗饲料　； 参考：《广西大学》2015年硕士论文

【摘要】：农作物秸秆提供了丰富的粗饲料来源,但由于诸多营养限制因素,不利于动物的消化吸收。本论文利用绿色木霉、M-2菌株和热带假丝酵母发酵不同秸秆饲料,对其营养成分变化进行研究,并用体外产气法对其营养价值进行评定,目的是研究不同菌种对秸秆营养成分的影响。主要实验结果如下：(1)绿色木霉和M-2菌株单独发酵六种不同秸秆饲料时,都具有较高的纤维素酶活性。M-2菌株在玉米秸秆、甘蔗梢、木薯渣、象草培养基上的纤维素酶活性较绿色木霉高。六种秸秆饲料接种绿色木霉和M-2菌株发酵后粗蛋白含量都有显著提高,半纤维素,纤维素都发生了一定的降解。(2)通过正交试验,确定M-2菌株和热带假丝酵母在木薯渣培养基上的最优发酵条件为：温度35℃,料水比1：1.6,pH为7,接种M-2菌株60 h后接种热带假丝酵母。在此最佳发酵条件下,M-2菌株和热带假丝酵母混合发酵木薯渣后粗蛋白含量、半纤维素降解率、纤维素降解率分别为21.89%、58.36%和41.91%；M-2菌株和热带假丝酵母在玉米秸秆培养基上的最优发酵条件为：温度35℃,料水比1：1.4,pH为6,接种木霉84h后接种酵母。在此最佳发酵条件下,M-2菌株和热带假丝酵母混合发酵玉米秸秆后粗蛋白含量、半纤维素降解率、纤维素降解率分别为19.65%、35.70%和20.73%。(3)采用体外产气法分别测定了不同发酵处理后秸秆饲料72h累计产气量并与处理前进行对照,结果表明,不同菌种发酵处理后秸秆饲料体外产气量均有下降。由24h的累计产气量计算得到各发酵秸秆饲料的有机物降解率、代谢能和消化能较对照组均有升高。
[Abstract]:Crop straw provides abundant forage sources, but it is not conducive to digestion and absorption of animals due to many nutritional constraints. In this paper, we used Trichoderma viride M-2 strain and Candida tropicalis to ferment different straw forage, and to study the change of nutrient composition and evaluate its nutritional value by in vitro gas production method. The aim of this study was to study the effect of different strains on the nutrient composition of straw. The main results were as follows: (1) Trichoderma viride and strain M-2 had higher cellulase activity in corn stalks, sugarcane shoots, cassava dregs. The cellulase activity in the medium was higher than that in Trichoderma viridis. After inoculation with Trichoderma viride and strain M-2, the crude protein content of six kinds of straw feed increased significantly, and hemicellulose and cellulose were all degraded. The optimum fermentation conditions of M-2 strain and Candida tropicalis on cassava dregs culture medium were determined as follows: temperature 35 鈩，

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