基于皮肤组织转录组数据研究水牛与黄牛温度适应性的差异

发布时间：2018-05-12 04:12

本文选题：转录组 + 信号通路　；参考：《中国畜牧兽医》2017年07期

【摘要】：为了找到与水牛和黄牛温度适应性差异相关的信号通路,本研究对3头成年水牛和3头成年黄牛的皮肤组织进行了转录组测序和生物信息学分析。结果显示,测序后水牛和黄牛皮肤组织分别得到59 369 167和69 837 009条reads,与参考基因组的唯一比对率分别为70.76%和75.55%。水牛和黄牛皮肤组织共有1 611个差异表达基因,其中801个在水牛皮肤中上调,810个在水牛皮肤中下调。GO分析结果显示,注释到细胞组分、分子功能、生物学过程的差异表达基因分别为316、263和278个。KEGG分析结果显示,差异表达基因共富集在33条信号通路上,其中神经活性配体—受体相互作用、氧化磷酸化、胆碱能突触、5-羟色胺能神经突触、GABA能突触、钙信号通路等信号通路可能与水牛和黄牛在温度适应性上的差异相关。综上所述,突触与氧化磷酸化可能与水牛和黄牛之间的温度适应性差异有关,本研究为进一步了解水牛和黄牛对温度适应性差异的分子机制和分子育种奠定基础。
[Abstract]:In order to find the signal pathway related to the temperature adaptation of buffalo and yellow cattle, the transcriptome sequencing and bioinformatics analysis of skin tissue of 3 adult buffalo and 3 adult cattle were carried out in this study. The results showed that 59,369,167 and 69,837,009 readswere obtained from the skin tissue of buffalo and yellow cattle, respectively, and the unique alignment rates with reference genome were 70.76% and 75.55%, respectively. There were 1 611 differentially expressed genes in buffalo and yellow cattle skin tissues, of which 801 were up-regulated in buffalo skin and 810 were down-regulated in buffalo skin. The results of down-regulation of .go in buffalo skin showed that the cell components and molecular functions were annotated. The differentially expressed genes in biological process were 316263 and 278.KEGG, respectively. The results showed that the differentially expressed genes were co-enriched in 33 signal pathways, in which neuroactive ligand-receptor interaction and oxidative phosphorylation were observed. Cholinergic synapse 5-hydroxytryptaminergic synapse GABA synapse and calcium signaling pathway may be related to the differences in temperature adaptation between buffalo and yellow cattle. In conclusion, synaptic and oxidative phosphorylation may be related to the difference of temperature adaptability between buffalo and yellow cattle. This study lays a foundation for further understanding the molecular mechanism and molecular breeding of temperature adaptation difference between buffalo and yellow cattle.
【作者单位】：内蒙古农业大学生命科学学院;中华人民共和国二连浩特出入境检验检疫局;
【基金】：内蒙古自然科学基金(2016MS0349) 内蒙古高等学校科学研究项目(NJZY16067)
【分类号】：S823

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