马疱疹病毒1型gB糖蛋白线性B细胞表位的预测与鉴定

发布时间：2018-05-12 07:52

本文选题：B细胞表位 + 抗原表位预测　；参考：《中国畜牧兽医》2017年05期

【摘要】：试验旨在应用生物信息学技术综合分析马疱疹病毒1型(equine herpesvirus 1,EHV-1)gB糖蛋白,预测B细胞表位,筛选出具有潜在诊断价值的线性B细胞表位。将EHV-1gB糖蛋白的基因序列输入DNAStar软件中的Protean工作区中,经参数综合比较分析筛选潜在的B细胞表位,克隆、表达预测表位的基因片段,利用表达的融合蛋白作为抗原与马疱疹病毒阳性血清反应。经预测分析,gB糖蛋白的B细胞表位可能位于第6—10、23—32、53—65、72—98、111—120、152—166和173—180位氨基酸区域。本试验成功构建并原核表达含7个潜在B细胞表位的融合蛋白。Western blotting试验结果显示,其中5个融合蛋白能被马疱疹病毒阳性血清识别。本试验利用生物信息学技术结合分子生物学技术成功筛选到5个潜在的B细胞表位,为EHV-1表位诊断、表位疫苗抗原的设计奠定了技术基础。
[Abstract]:The purpose of the experiment was to comprehensively analyze the EHV-1gB glycoprotein of equine herpesvirus 1 by using bioinformatics technique, to predict the B cell epitopes and to screen the linear B cell epitopes with potential diagnostic value. The gene sequence of EHV-1gB glycoprotein was input into the Protean workspace of DNAStar software. The potential B cell epitopes were screened, cloned, and the predicted epitope gene fragments were expressed by comprehensive analysis of parameters. The expressed fusion protein was used as antigen to react with equine herpesvirus positive serum. It is predicted that the B cell epitopes of G B glycoprotein may be located in the amino acid regions of the 6-10m23 ~ 32N _ 53-65N _ 72-98111-120152-166 and 173-180 amino acids. The results of Western blotting assay showed that five of the fusion proteins could be recognized by equine herpesvirus positive serum. In this study, five potential B cell epitopes were successfully screened by bioinformatics and molecular biology, which laid a technical foundation for the diagnosis of EHV-1 epitopes and the design of epitope vaccine antigens.
【作者单位】：新疆农业大学动物医学学院;
【基金】：国家科技支撑计划项目(2012BAD46B04-03)
【分类号】：S852.652

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