山羊BMPR-IB基因密码子偏好性及聚类分析

发布时间：2018-05-15 07:59

本文选题：BMPR-IB + 山羊　；参考：《生物技术》2017年02期

【摘要】：[目的]利用生物信息学分析山羊BMPR-IB基因密码子使用特征,对不同物种BMPR-IB基因通过不同的聚类方法进行分析。[方法]利用Usage Codon在线程序和Codon W软件分析山羊和其他物种BMPR-IB基因对密码子偏好性的使用情况,通过欧式平方距离和最小进化法分别进行聚类分析。[结果]山羊BMPR-IB基因无G/C碱基的使用倾向,GCC、CTG、CAG、CCC、AGA、AGT、GTG和TGA为山羊BMPR-IB基因的偏好密码子,其余53种密码子的使用较为均衡。通过最小进化法建立起来的不同物种间的系统发育分析结果与动物学分类一致,且不同物种BMPR-IB基因编码蛋白表达水平存在种属差异。[结论]BMPR-IB基因偏爱使用以A或T结尾的密码子,基于欧式距离系数建立起来的聚类和最大似然法构建的聚类不一致,造成这种差异的原因可能是在进化过程中单基因突变所引起的。
[Abstract]:[objective] to analyze the codon usage characteristics of goat BMPR-IB gene by bioinformatics, and to analyze BMPR-IB gene of different species by different clustering methods. [methods] the use of codon preference of goat and other species BMPR-IB gene was analyzed by Usage Codon online program and Codon W software, and cluster analysis was carried out by Euclidean square distance and minimal evolution method. [results] there was no G / C base in goat BMPR-IB gene. The GCC-CTG, CAGCCC, AGAAGTG, GTG and TGA were the preferred codon of goat BMPR-IB gene, and the other 53 codon were more balanced. The phylogenetic analysis of different species established by minimum evolution method was consistent with zoological classification, and the expression level of BMPR-IB gene coding protein in different species was different in species. [conclusion] the codon ending in A or T is preferred by BMPR-IB gene. The clustering based on Euclidean distance coefficient is inconsistent with that constructed by maximum likelihood method. The difference may be caused by a single gene mutation during evolution.
【作者单位】：河北农业大学动物科技学院;
【基金】：国家现代农业产业技术体系项目-绒毛用羊产业技术体系(“绒毛用羊关键基因发掘及其基因编辑研究”,No.CARS-40-04B)
【分类号】：Q78;S827

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