野生和栽培木薯叶片的营养及饲料价值研究

发布时间：2018-05-15 08:21

本文选题：木薯 + 野生种　；参考：《南方农业学报》2017年02期

【摘要】：【目的】研究不同木薯种质叶片的营养成分差异,为木薯叶作为饲料资源开发利用提供参考。【方法】测定8个木薯种质(3个野生种和5个栽培种)功能叶片的粗脂肪、粗蛋白、可溶性糖、淀粉、类胡萝卜素和氢氰酸(HCN)含量,并利用Western blotting测定功能叶片相关蛋白酶(UGPase、AGPase、GBSSI和Linamarase)的表达水平。【结果】8个木薯种质中,野生种Manihot cecropiaefolia Phhl功能叶片的淀粉、可溶性糖和类胡萝卜含量最高,分别为2.5 g/100 g DW、30.0 g/100 g DW和91.9 mg/100 g FW,蛋白质和HCN含量中等;栽培种SC9功能叶片蛋白质和可溶性糖含量中等,HCN含量最低,仅167 mg/kg FW。Western blotting分析结果表明,M.cecropiaefolia Phhl叶片糖和淀粉代谢通路的关键酶UGPase、AGPase和GBSSI表达水平最高,SC9 HCN代谢通路的关键酶Linamarase表达水平最低,各蛋白质表达水平与相关生理指标变化趋势一致。通过主成分分析结果可看出,叶片淀粉和可溶性糖含量较高的木薯种质,粗脂肪含量较低;可溶性糖、粗蛋白和类胡萝卜素含量较高的木薯种质,HCN含量较低。【结论】野生木薯M.cecropiaefolia Phhl完全展开功能叶片的综合营养价值较高,可考虑作为畜禽和蚕用饲料资源开发利用;栽培种木薯SC9新鲜叶片HCN含量较低,也可优先作为蚕用饲料。
[Abstract]:[objective] to study the difference of nutrient components in leaves of cassava germplasm, and to provide a reference for the exploitation and utilization of cassava leaves as feed resources. [methods] the crude fat of functional leaves of 8 cassava germplasms (3 wild species and 5 cultivated species) was determined. The contents of crude protein, soluble sugar, starch, carotenoid and hydrocyanoate were determined by Western blotting. The expression levels of Manihot cecropiaefolia Phhl functional leaves in 8 cassava germplasms were determined by Western blotting. The contents of soluble sugar and carrot were the highest, which were 30.0 g / 100 g DW and 91.9 mg/100 g FW, respectively, and the contents of protein and HCN were moderate, and the content of protein and soluble sugar in SC9 functional leaves was the lowest. Only 167 mg/kg FW.Western blotting analysis showed that the key enzymes of sugar and starch metabolism pathway of M. cecropiaefolia Phhl were UGPase and GBSSI. The expression level of Linamarase was the lowest in SC9 HCN metabolic pathway, and the protein expression level was consistent with the change trend of related physiological indexes. The results of principal component analysis showed that cassava germplasm with higher content of starch and soluble sugar in leaves had lower crude fat content and soluble sugar, The cassava germplasm with higher crude protein and carotenoid content had lower HCN content. [conclusion] the comprehensive nutritional value of the fully expanded leaves of wild cassava M.cecropiaefolia Phhl was higher, which could be considered as a feed resource for livestock, poultry and silkworm. The content of HCN in fresh leaves of cassava SC9 is low, and it can also be used as silkworm feed.
【作者单位】：中国热带农业科学院热带作物品种资源研究所/农业部木薯种质资源保护与利用重点实验室;贵州省亚热带作物研究所;华中农业大学植物科学技术学院;Natural
【基金】：海南省创新创业人才启动基金——中英非研究挑战基金项目(Agri TT Research Challenge Fund,Reference:1561) 中国热带农业科学院科技攻关专题项目(1630032015005)
【分类号】：S816

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