硫磺负离子添加剂对大鼠的亚慢性毒性试验

发布时间：2018-05-15 18:20

本文选题：硫磺负离子 + 大鼠　；参考：《延边大学》2015年硕士论文

【摘要】：本试验主要研究硫磺负离子饲料添加剂对大鼠的亚慢性毒性和对肝脏抗氧化能力的影响。选取清洁级SD大鼠80只,雌雄各半。随机分成4组,每组雌雄各10只,分别为对照组,饲喂普通饲料；低剂量组,饲喂含有0.3%硫磺负离子饲料添加剂的饲料；中剂量组,饲喂含有0.9%硫磺负离子饲料添加剂的饲料；高剂量组,饲喂含有2.7%硫磺负离子饲料添加剂的饲料。连续喂养90d。第45天每组挑选6只大鼠,雌雄各3只,进行采血、剖检、脏器称重。第90d,所有大鼠全部采血、剖检、脏器称重、并将肝脏研磨测定丙二醛(MDA)、谷胱甘肽(GSH)、过氧化物歧化酶SOD比活力、对高剂量组大鼠肾脏作组织学观察。试验结果：(1)大鼠体重的变化：试验期间各组动物的基本情况良好,无厌食、死亡和其它异常行为,通过每周对大鼠体重的记录,发现雌性高剂量组在第28天和第49天时与对照组有显著性差异(P0.05),其余各组与对照组相比较无显著性差异。(2)饲料利用率：硫磺负离子饲料添加剂饲喂初期,试验组饲料利用率明显低于对照组,随着试验的进行,饲料利用率在不断提高,到第六周试验组的饲料利用率开始高于对照组。(3)脏器系数：试验中期高剂量组雌性大鼠的肺的脏器系数高于对照组,并且差异显著(p0.05),其余各组与对照组相比较均无显著性差异；试验末期低剂量组雄性大鼠的肾脏的脏器系数与对照组相比有显著性差异(p0.05),中剂量组雌性大鼠胸腺的脏器系数与对照组相比有显著性差异(p0.05),其余各试验组与对照组相比较均无显著性差异(4)血液学检查结果：硫磺负离子90 d喂养试验雌雄性大鼠中期和末期低、中、高三个剂量组的血液学检查结果与对照组相比较均无显著性差异。(5)血清生化检查结果：试验中期雌性低剂量组和高剂量组的谷草转氨酶明显偏高,与对照组比较差异极显著(p0.01),雄性高剂量组碱性磷酸酶水平偏高,与对照组比较有显著性差异(p0.05),；试验末期雌雄大鼠各个试验组的血清学检查结果与对照组相比均无显著性差异。(6)组织学检查结果：通过光学显微镜对组织切片的观察,未发现组织有异常变化。(7)肝脏抗氧化能力：对大鼠肝脏中丙二醛和谷胱甘肽的含量没有明显的影响,与对照组相比较没有产生显著性的差异(p0.05)；对肝脏过氧化物歧化酶(SOD)比活力也有明显的影响,试验组各组的SOD比活力比对照组高,中剂量组有统计学差异,(p0.05)。本试验结果表明硫磺负离子饲料添加剂喂养大鼠90d没有产生毒性,并且能够增强大鼠肝脏抗氧化能力,本试验中的最适添加量为0.9%。
[Abstract]:The effects of sulfur anion feed additive on the subchronic toxicity and antioxidation ability of liver in rats were studied. Eighty SD rats of clean grade were selected, half male and half male. They were randomly divided into 4 groups: control group (n = 10), low dose group (fed with 0.3% sulfur negative ion), medium dose group (n = 10), and medium dose group (n = 10) fed with 0.9% sulfur negative ion feed additive. High dose group, feed containing 2.7% sulfur negative ion feed additive. Continuous feeding for 90 days. On the 45th day, 6 rats, male and female, were selected for blood collection, dissection and weighing of viscera. On the 90th day, all the rats were collected blood, dissected, weighed by viscera, and the specific activities of malondialdehyde (MDA), glutathione (GSH) and peroxidase dismutase (SOD) were measured by liver grinding. The kidney of high dose group was observed histologically. Results: changes in the body weight of rats: during the trial, the animals in each group were basically in good condition, free of anorexia, death and other abnormal behaviors, and recorded the weight of the rats on a weekly basis. It was found that there was significant difference between the female high dose group and the control group on the 28th and 49th day, but there was no significant difference between the other groups and the control group. The feed utilization rate of the test group was obviously lower than that of the control group. At the sixth week, the feed utilization ratio of the experimental group was higher than that of the control group. The organ coefficient of the female rats in the high dose group was higher than that in the control group, and the difference was significant (p 0.05). There was no significant difference between the other groups and the control group. At the end of the experiment, the kidney organ coefficient of the male rats in the low dose group was significantly different from that of the control group (p 0.05), the thymus organ coefficient of the female rats in the middle dose group was significantly different from that of the control group, and the other test groups were different from the control group. There was no significant difference between the two groups (4) the results of hematological examination showed that the middle and late stages of female and male rats fed with sulfur anion for 90 days were lower than those in the control group. There was no significant difference in serum biochemistry between the three dose groups and the control group. The results of serum biochemical examination showed that the glutamic oxaloacetic transaminase was significantly higher in the female low dose group and high dose group in the middle of the trial. Compared with the control group, the difference was significant (P 0.01), and the alkaline phosphatase level in the male high dose group was higher than that in the control group. At the end of the experiment, there was no significant difference in serological examination results between male and female rats compared with the control group. No abnormal changes were found in the tissue. 7) the antioxidant capacity of liver: there was no significant effect on the contents of malondialdehyde and glutathione in the liver of rats. Compared with the control group, there was no significant difference between the two groups (p0.05), and the specific activity of SOD in liver was also significantly affected. The specific activity of SOD in the experimental group was higher than that in the control group, and there was a significant difference in the middle dose group (P 0.05). The results showed that sulfur negative feed additive did not produce toxicity for 90 days and could enhance the anti-oxidation ability of rat liver. The optimum dosage was 0.9g in this experiment.
【学位授予单位】：延边大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S859.82

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