藏猪粪样微生物对不同纤维底物的体外发酵

发布时间：2018-05-17 04:30

本文选题：藏猪 + 产气量　；参考：《中国兽医学报》2017年07期

【摘要】：试验选用藏猪(n=5)新鲜粪样作为发酵接种物,以小麦、豌豆、燕麦和油菜秸秆为纤维底物分别进行体外发酵,测定产气量、发酵液中挥发性脂肪酸(volatile fatty acid,VFA)和铵态氮(NH3-N)的含量以及各纤维底物中纤维素和半纤维素降解率。结果显示:经发酵,各底物中纤维素和半纤维素的含量显著降低,豌豆秸秆中半纤维素降解率显著高于其他3种底物;豌豆秸秆产气量最高,且理论最大产气量显著高于其他3种底物,油菜秸秆的产气延滞时间显著低于其他3种底物;各底物发酵液中,乙酸和丙酸含量最多,且豌豆秸秆乙酸含量显著高于其他3种底物,而燕麦秸秆丙酸含量显著高于其他3种底物。各底物发酵液中铵态氮质量浓度为7.09~11.53g/L。结果表明:藏猪肠道微生物能够分解粗纤维,并且对豌豆秸秆的利用率较高,为藏猪非常规饲料资源的开发和利用提供理论依据。
[Abstract]:Fresh manure samples were used as inoculants. Wheat, pea, oat and rape straw were used as fiber substrates for in vitro fermentation to determine gas production. The contents of volatile fatty acid volatile fatty acidine (VFA) and ammonium nitrogen (NH3-N) in fermentation broth, and the degradation rates of cellulose and hemicellulose in each fiber substrate. The results showed that the content of cellulose and hemicellulose in each substrate decreased significantly, the degradation rate of hemicellulose in pea straw was significantly higher than that in the other three substrates, and the gas production of pea straw was the highest. The theoretical maximum gas production was significantly higher than that of the other three substrates, and the delay time of rape straw production was significantly lower than that of the other three substrates, the content of acetic acid and propionic acid was the highest in the fermentation broth of each substrate. The acetic acid content of pea straw was significantly higher than that of the other three substrates, while the propionic acid content of oat straw was significantly higher than that of the other three substrates. The mass concentration of ammonium nitrogen in the fermentation broth was 7.09 g / L and 11.53 g / L. The results showed that intestinal microorganism could decompose the crude fiber, and the utilization rate of pea straw was higher, which provided the theoretical basis for the exploitation and utilization of the unconventional feed resources of Tibetan pigs.
【作者单位】：西藏农牧学院动物科学学院;西北农林科技大学动物科技学院;华中农业大学动物医学院;
【基金】：西藏自治区科技厅重点资助项目“藏东南地区藏猪主要传染病调查及防控研究” 国家“十二五”科技支撑计划资助项目(2012BAD3B03)
【分类号】：S828

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