复方中药超微粉对东乡黑羽绿壳蛋鸡脂类代谢的影响

发布时间：2018-05-17 06:20

本文选题：东乡黑羽绿壳蛋鸡 + 中药超微粉　；参考：《江西农业大学》2015年硕士论文

【摘要】：本试验以东乡黑羽绿壳蛋鸡为研究对象,通过在其日粮中添加不同的复方中药超微粉,探究复方中药超微粉对东乡黑羽绿壳蛋鸡生产性能、脂类代谢及apo AⅠ、apo B100 m RNA表达量的影响。试验选用22周龄的健康东乡黑羽绿壳蛋鸡135羽,随机分为3个组:对照组饲喂基础日粮,试验1组饲喂基础日粮+配方Ⅰ,试验2组饲喂基础日粮+配方Ⅱ。试验期60天,每隔15天对各组进行宰杀采样,测定相关指标并对结果进行统计分析。试验主要结果如下:1.,试验1组、试验2组鸡蛋胆固醇的含量,与对照组相比显著降低,试验的第60d降幅分别为35.66%(P0.01)和28.07%(P0.01);2.与对照组比较,试验1组、试验2组均能显著降低肝脏中粗脂肪的含量(P0.05);试验1组对胸肌和腿肌中的粗脂肪含量也有显著的降低作用(P0.05),同时试验2组对胸肌和腿肌中粗脂肪的含量也有降低作用,但差异不显著(P0.05)。3.与对照组比较,试验1组、试验2组均能显著降低血液中总胆固醇脂的含量(P0.05),试验1组能显著降低低密度胆固醇脂的含量(P0.05),试验2组对低密度胆固醇酯有降低作用(P0.05);试验1组能显著升高高密度胆固醇脂的含量(P0.05),试验2组也能升高血液中高密度胆固醇酯的含量,但差异不显著(P0.05);4.试验第60天,试验1组、试验2组apo AⅠm RNA在肝脏中的表达量与对照组比较均显著升高(P0.05);肾脏和小肠中的表达量有不同程度的升高,但差异不显著(P0.05);与对照组比较,试验1组、试验2组肾脏中apo B100 m RNA表达量升高,差异显著(P0.05),肝脏与小肠中的apo B m RNA表达量也有升高,但差异不显著(P0.05)。上述结果表明,两种不同配方的复方中药超微粉制剂均能明显的降低蛋鸡鸡蛋中的胆固醇含量;上调蛋鸡体内的apo AⅠm RNA和apo B100 m RNA表达水平;对蛋鸡体内的脂类代谢的调节有一定的作用,且配方Ⅰ制剂的调节作用优于配方Ⅱ制剂。
[Abstract]:In this experiment, Dongxiang black feather green shell laying hens were taken as the research object. By adding different compound traditional Chinese medicine ultrafine powder in the diet, the effect of compound traditional Chinese medicine ultrafine powder on the production performance, lipid metabolism and the expression of apo A I and apo B100 m RNA in the black feather green shell chicken were investigated. The experiment selected 135 plumes of the healthy Dongxiang black plume green shell laying hens with 22 weeks old age. The control group was divided into 3 groups: the control group was fed with the basal diet, the experiment 1 groups were fed with basic diet + formula I, the test 2 groups were fed with basal diet + formula II. The test period was 60 days and every 15 days were slaughtered, the related indexes were measured and the results were statistically analyzed. The main results were as follows: 1., test 1 groups, and test 2 groups of egg cholesterol content Compared with the control group, the decrease of 60d was 35.66% (P0.01) and 28.07% (P0.01), respectively. 2. compared with the control group, the 1 groups and the 2 groups could significantly reduce the content of crude fat in the liver (P0.05), and the 1 groups also significantly decreased the crude fat content in the chest and leg muscles (P0.05), and the 2 groups were tested on the chest muscles and in the 2 groups. The content of crude fat in the leg muscles also decreased, but the difference was not significant (P0.05).3. compared with the control group, the test 1 groups, the test 2 groups can significantly reduce the content of total cholesterol in the blood (P0.05), the 1 groups can significantly reduce the content of low density cholesterol fat (P0.05), the test of low density cholesterol esters have decreased (P0.05); test The 1 groups could significantly increase the content of high density cholesterol fat (P0.05), and the 2 groups could also increase the content of high density cholesterol esters in the blood, but the difference was not significant (P0.05); 4. test sixtieth days, test 1, the expression of apo A I m RNA in the liver was significantly higher than that of the control group (P0.05); the expression in the kidneys and small intestine was not The difference was not significant (P0.05) in the same degree (P0.05). Compared with the control group, the expression of apo B100 m RNA in the kidneys of the test group was increased, the difference was significant (P0.05), the apo B m RNA expression in the liver and the small intestine also increased, but the difference was not significant (P0.05). The results showed that the two different formulations of the compound Chinese medicine ultrafine powder were all capable of The level of apo A I m RNA and apo B100 m RNA expression level in laying hens was obviously reduced, and the regulation of lipid metabolism in laying hens had a certain effect, and the regulation effect of formula I preparation was better than that of formula II preparation.
【学位授予单位】：江西农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S831

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