饮水添加硼不影响乌鸡骨骼肌品质

发布时间：2018-05-17 12:56

本文选题：硼 + 乌鸡　；参考：《中国组织化学与细胞化学杂志》2016年03期

【摘要】：目的明确饮水中添加硼对乌鸡骨骼肌品质的影响。方法1日龄乌鸡24羽,随机分成6组,每组4羽,饲喂相同的基础日粮及含不同浓度硼(0、40、80、160、320、640mg/L)的饮水,80d后,取乌鸡髂腓肌作为实验样品。用石蜡切片免疫组织化学检测骨骼肌Bcl-2表达;测定组织pH值、水分、灰分、粗蛋白、粗脂肪、剪切力、肉色及滴水损失。结果除在饲喂640mg/L乌鸡骨骼肌中,Bcl-2免疫组织化学表达上调,pH值、灰分显著性升高,粗蛋白含量显著性降低外,水分、粗脂肪、剪切力、肉色及滴水损失在各浓度饲喂乌鸡骨骼肌中均无明显差异。结论结果表明,乌鸡肌肉品质各项指标对饮水中硼的浓度不敏感,只有达到极高剂量(640mg/L)时才会使pH值、灰分、粗蛋白发生显著性变化,这些变化可能与骨骼肌细胞Bcl-2的表达有关。
[Abstract]:Objective to investigate the effect of boron addition in drinking water on skeletal muscle quality of black chicken. Methods Twenty-four silky chickens of one day old were randomly divided into 6 groups (4 in each group) and fed with the same basal diet and drinking water containing different concentrations of boron (40,8016032040mg / L) for 80 days. The iliofibular muscles were taken as experimental samples. The expression of Bcl-2 in skeletal muscle was detected by immunohistochemical method in paraffin section, pH value, water content, ash, crude protein, crude fat, shear stress, meat color and drop water loss were measured. Results except for the immunohistochemical expression of Bcl-2 in skeletal muscle of 640mg/L chickens, the expression of Bcl-2 was up-regulated, the ash content was significantly increased, and the content of crude protein was significantly decreased, water, crude fat, shear stress were also significantly decreased. There was no significant difference in meat color and drip loss in the skeletal muscles of silky chickens fed with different concentrations. Conclusion the results showed that the indexes of muscle quality of black chicken were not sensitive to the concentration of boron in drinking water, and the pH, ash and crude protein changed significantly only when the concentration of boron in drinking water reached 640mg / L. These changes may be related to the expression of Bcl-2 in skeletal muscle cells.
【作者单位】：厦门医学院基础部;福建卫生职业技术学院基础部;福州市第二医院;
【基金】：福州市科学技术局(2014-G-57)
【分类号】：S831.5

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