鸡传染性腺胃炎的免疫学研究

发布时间：2018-05-17 23:11

本文选题：鸡传染性腺胃炎 + 流行病学　；参考：《聊城大学》2015年硕士论文

【摘要】：鸡传染性腺胃炎是一种由多种原因引起的以腺胃肿大、腺胃乳头出血溃疡为重要特征的慢性消化道性的传染病,是危害养禽业的重要疾病之一。本试验先对其流行病学进行调查研究,后从机体免疫学角度入手,研究鸡传染性腺胃炎发生过程中7种不同细胞因子的变化情况;采用MTT法对21日龄的发病鸡进行外周血淋巴细胞的分离、培养和增殖反应活性检测,研究感染鸡传染性腺胃炎后机体内新城疫和禽流感抗体的变化情况;观察该病的组织病理学变化,了解鸡传染性腺胃炎的发病机制,为鸡传染性腺胃炎的有效防控奠定基础。1.鸡传染性腺胃炎的流行病学调查鸡传染性腺胃炎是危害养禽业的主要疾病之一,主要症状为采食量下降,生长缓慢,腺胃肿大,腺胃乳头粘膜脱落、出血甚至溃疡等。该病主要侵害3-25d的鸡群,病死率高达30%,病程较长,可持续10-15d。12个不同地区同一时间的鸡群均发生鸡传染性腺胃炎,表明该病在不排除疫苗污染的情况下传播方式可能是垂直传播。2.感染鸡传染性腺胃炎后机体内细胞因子含量的变化采用酶联免疫吸附试验(ELISA)对发病组和健康组的鸡群采样进行细胞因子的测定,发现感染鸡传染性腺胃炎后的鸡群体内IL-1、IL-6、IL-8、TNF-ɑ、SIg A等细胞因子的含量高于健康鸡群,而IL-2和IFN-γ的含量低于健康鸡群。在病变最典型的21d,感染鸡传染性腺胃炎的鸡群体内细胞因子的含量变化最为显著,结果表明鸡群感染传染性腺胃炎后出现明显的炎症反应,导致与免疫相关的细胞因子发生相应的变化。3.感染鸡传染性腺胃炎对机体免疫应答的影响通过MTT法测定21d鸡外周血淋巴细胞的增殖反应,发现发病鸡机体内T淋巴细胞浓度高于健康对照组,但差异性不显著。通过HA和HI法对鸡新城疫和禽流感抗体水平进行测定,发现健康鸡的抗体水平在14d时,禽流感H9抗体水平明显高于发病鸡,其余时间差异不显著,而新城疫抗体水平差异性不显著,所以鸡传染性腺胃炎的发生对机体的疫苗免疫反应有选择性的部分抑制作用。4.鸡传染性腺胃炎的病理组织学观察制备相关器官(如腺胃、心脏、肝脏、脾脏、肾脏等)的病理组织切片,通过HE染色,观察其病理组织学变化。结果表明,患病鸡的腺胃、心脏、肝脏、脾脏等器官有明显的出血,并伴有大量的炎性细胞浸润,腺胃固有层、黏膜下层等不同部位都出现水肿,同时出现腺胃乳头出血,腺小管间淤血等现象。这些病理组织学变化与临床出现腺胃明显肿大、腺胃乳头出血、溃疡等剖检特征相一致。
[Abstract]:Infectious glandular gastritis is a chronic digestive tract infectious disease which is caused by a variety of causes, such as adeno and gastric enlargement and gastric papillary bleeding and ulcer, which is one of the most important diseases that harm the poultry industry. This experiment first investigated the epidemiology of the disease, and then began to study the occurrence of infectious glandular gastritis from the point of immunity from the organism. The change of 7 different cytokines in the process, the MTT method was used to separate the peripheral blood lymphocytes of the 21 day old chickens, to detect the activity of the culture and proliferation reaction, to study the changes of the Newcastle disease and the avian influenza antibody in the infected chicken infectious gland gastritis, and to observe the histopathological changes of the disease and to understand the infectious gonadal gland of the chicken. The pathogenesis of gastritis, laying the foundation for the effective prevention and control of infectious glandular gastritis, the epidemiological investigation of infectious adenogastritis in chicken.1., chicken infectious adenogastritis is one of the main diseases endangering the poultry industry. The main symptoms are the decline in intake, slow growth, glandular stomach swelling, glandular mucous mucous membrane exfoliation, bleeding even ulcers. The fatality rate is up to 30% and the course of disease is long. The chicken infective gastritis of chickens in different regions of the same time in different regions of the 10-15d.12 can be infected by 3-25d, which indicates that the transmission mode of the disease may be the change of the cytokine content in the body of infectious glandular gastritis in the vertical transmission of.2. infection without excluding the contamination of the vaccine. The immuno adsorption test (ELISA) was used to determine the cytokines in the chickens of the disease group and the healthy group. It was found that the contents of IL-1, IL-6, IL-8, TNF-, and SIg A in the chickens infected with infectious chicken gastritis were higher than those of healthy chickens, but the content of IL-2 and IFN- gamma was lower than that of the healthy chickens. The infection of chicken in the most typical 21d of the disease was infected with chickens. The changes in the content of cytokines in the chicken group of gonadal gastritis were the most significant. The results showed that the chickens infected with infectious gonadal gastritis had obvious inflammatory response, which resulted in the corresponding changes in the immune related cytokines. The effect of.3. infection on the immune response of chicken infectious glandular gastritis was determined by MTT method for the determination of the peripheral blood lymph of 21d chickens. The proliferation reaction of the cell was found to be higher than that in the healthy control group, but the difference was not significant. The antibody level of Newcastle disease and avian influenza was measured by HA and HI, and the antibody level of the healthy chicken was higher than that of the disease chicken when the antibody level of the healthy chicken was at 14d. The difference of the rest time was not significant, but the new Newcastle disease was not significant. The difference of antibody level is not significant, so the occurrence of avian infectious adenogastritis has a selective partial inhibitory effect on the immune response of the body. The histopathological sections of the related organs (such as the stomach, heart, liver, spleen, kidney, etc.) of.4. infectious glandular gastritis (such as adeno stomach, heart, liver, spleen, kidney and so on) are prepared, and the pathological histology is observed by HE staining. The results showed that the glandular stomach, heart, liver, spleen and other organs of the sick chicken had obvious bleeding, accompanied by a large number of inflammatory cell infiltration, edema in the propria of the glands and the submucosa and other parts of the submucosa, and the phenomena of glandular gastric papilla bleeding and glandular intertubule congestion. The features of large, bleeding of gastric glands, bleeding and ulcer were consistent.
【学位授予单位】：聊城大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S858.31

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