2012-2013年河南省部分规模化猪场PRV野毒感染调查与净化结果分析

发布时间：2018-05-18 23:04

本文选题：PRV + 抗体监测　；参考：《南京农业大学》2015年硕士论文

【摘要】：猪伪狂犬病由伪狂犬病毒引起,近年来,该病给我国养猪业造成了巨大的经济损失,特别是近几年PRV野毒的感染在规模化猪场有上升趋势,同时,PRV强毒株的出现,给疫苗免疫提出了很大的挑战。当前,市场上主要使用猪伪狂犬病毒gE基因缺失疫苗进行猪群的PRV疫苗免疫,PRVgE抗体检测试剂盒可用于PRV野毒感染抗体的检测。本研究使用当前市场使用最为广泛的试剂盒是美国IDEXX公司生产的PRV gE抗体检测试剂盒对河南省43家规模化猪场进行PRV野毒感染评估,并对其中的5个规模化猪场进行了重点监测。除重点监测的5个场外的其它养殖场在2012年共送检26批次样品,其中PRV gE抗体阳性的有19批次,占73.1%;2013年共送检39批次样品,其中PRVgE抗体阳性的有24批次,占61.5%。整体结果显示,河南地区的规模化猪场PRV野毒感染阳性率仍然很高,规模化猪场之间的生产管理水平和PRV防控措施产生的效果也差异巨大。重点检测的5个场,通过背景信息、免疫、监测和生物安全等方面分析和讨论,总结出了 PRV防控存在的问题和一些有效的防控措施。本研究也根据监测和评估结果对当前规模化猪场的PRV野毒感染及净化的问题进行了分析,提出了 PRV野毒净化的措施和建议.
[Abstract]:Porcine pseudorabies is caused by pseudorabies virus. In recent years, the disease has caused huge economic losses to the pig industry in China, especially in recent years, the infection of PRV wild virus has an increasing trend in large-scale pig farms, and the emergence of virulent strains of PRV. It poses a great challenge to vaccine immunization. At present, porcine pseudorabies virus GE gene deletion vaccine is mainly used in the market to detect PRV vaccine and PRVgE antibody of swine population. It can be used to detect the antibody against PRV wild virus infection. In this study, the most widely used kit in the market was the PRV GE antibody kit produced by IDEXX Company in the United States to evaluate the PRV field virus infection in 43 large-scale pig farms in Henan Province. Five of them were mainly monitored. In 2012, 26 batches of samples were collected from other farms except the 5 sites under special surveillance, of which 19 batches were positive for PRV GE antibody, accounting for 73.1 batches, and 39 batches of samples were tested in 2013, of which 24 batches were positive for PRVgE antibody, accounting for 61.5 batches. The overall results showed that the positive rate of PRV infection in large-scale pig farms in Henan Province was still very high, and the level of production management and the effect of PRV prevention and control measures among large-scale pig farms were also greatly different. Through the analysis and discussion of background information, immunization, surveillance and biosafety, the existing problems and some effective prevention and control measures of PRV were summarized. Based on the results of monitoring and evaluation, the problems of PRV field virus infection and purification in large scale pig farms were analyzed, and the measures and suggestions of PRV field virus purification were put forward.
【学位授予单位】：南京农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S858.28

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