峨眉雪胆微丸的制备及对断奶仔猪免疫功能和抗氧化性能的影响

发布时间：2018-05-18 22:30

本文选题：峨眉雪胆微丸 + 仔猪断奶　；参考：《四川农业大学》2015年硕士论文

【摘要】：养猪行业长期依靠抗生素来控制仔猪断奶应激综合征,但是抗生素会引起仔猪肠道微生物菌群失调和肠道发育受损等危害。与在饲粮中添加抗生素相比,添加中草药提取物,能有效地调节断奶仔猪消化道微生物体系的平衡,促进仔猪肠道发育,从而有效降低腹泻率,减少断奶应激反应,有助于提高仔猪的免疫力和生产性能。峨眉雪胆对腹泻的治疗效果较好,但在养猪生产上的研究还未见报道。因此,本研究探索了峨眉雪胆作为饲料添加剂对仔猪断奶应激综合征的预防效果。因峨眉雪胆味极苦,直接在饲料中添加会影响仔猪的适口性,因而将其制备成微丸。同时,研究了峨眉雪胆微丸对断奶仔猪免疫功能、血清生化指标和抗氧化活性的影响。结果如下：(1)峨眉雪胆微丸的制备：采用挤出滚圆一流化床包衣法对其处方条件和工艺条件进行考察,主要辅料为微晶纤维素(MCC),挤出转速为40 r/min,滚圆转速为400r/min,滚圆时间为30s,干燥后经流化床包衣制得峨眉雪胆微丸(1 g微丸含原雪胆生药1 g),圆整度、流动性、崩解时限均较理想,其工艺简便,成品质量好。(2)峨眉雪胆微丸对断奶仔猪免疫功能的影响：与抗生素组相比,断奶仔猪日粮中添加0.025%峨眉雪胆微丸使断奶仔猪血清免疫球蛋白IgG、IgM、IgA的含量(P0.05)显著提高,补体C3、C4的生成量增加以及细胞因子IL-6的分泌量增多(P0.05),而细胞因子IL-1β含量显著降低(P0.05)。并且能显著提高十二指肠、回肠、盲肠的分泌型免疫球蛋白A(SIgA)的分泌(P0.05)。结果表明：日粮中添加0.025%峨眉雪胆微丸可增强断奶仔猪的免疫功能。(3)峨眉雪胆微丸对断奶仔猪血清生化指标的影响：与抗生素组相比,断奶仔猪日粮中添加0.025%峨眉雪胆微丸能够使仔猪血清中总蛋白(TP),白蛋白(ALB)和球蛋白(GLB)的浓度增大,二者在第三周差异显著。血清中尿素氮的浓度显著降低。结果表明：峨眉雪胆微丸可改善断奶仔猪血清生化指标。(4)峨眉雪胆微丸对断奶仔猪抗氧化性能的影响：0.025%峨眉雪胆微丸能够显著提高血清SOD活性(P0.05),且影响持续时间长；能够提高仔猪GSH活性；在一定程度上降低MDA含量。结果表明：峨眉雪胆微丸作为中草药饲料添加剂能够增强断奶仔猪的抗氧化能力。
[Abstract]:For a long time, the pig industry relies on antibiotics to control weaning stress syndrome in piglets, but antibiotics can cause intestinal microflora imbalance and intestinal development damage in piglets. Compared with the supplement of antibiotics in diet, the addition of Chinese herbal medicine extract can effectively regulate the balance of digestive tract microbial system, promote the development of intestinal tract of weaning piglets, thus effectively reduce the diarrhea rate and stress response of weaning piglets. It is helpful to improve the immunity and production performance of piglets. Emei Xue gallbladder is effective in treating diarrhea, but the research on pig production has not been reported. Therefore, the preventive effect of Emei Xue gallbladder as a feed additive on weaning stress syndrome in piglets was investigated in this study. Due to the bitter taste of Emei snow gallbladder, adding directly to the feed will affect the palatability of piglets, so it is prepared into pellets. At the same time, the effects of Emei Xuedan pellet on immune function, serum biochemical index and antioxidant activity of weaned piglets were studied. The results were as follows: 1) the preparation of Emei Xuedan pellet: the prescription and technological conditions of the pellet were investigated by extrusion round and fluidized bed coating method. The main excipients were microcrystalline cellulose (MCC), the extrusion speed was 40 r / min, the rotary speed was 400 r / min, and the rolling time was 30 s. After drying and coating with fluidized bed, the Emei snow bile pellet was prepared by fluidized bed coating. The effect of Emei Xuedan pellet on the immune function of weaning piglets was simple and the quality of finished product was good. Compared with the antibiotic group, adding 0.025% Emei Xuedan pellet to the diet of weaning piglets could significantly increase the content of IgGG IgMMA IgA in the serum of weaned piglets (P0.05), compared with the antibiotic group, Emei Xuedan pellet added 0.025% Emei Xuedan pellets to the diet of weaning piglets. The production of complement C _ 3N _ 4 and the secretion of cytokine IL-6 were increased (P 0.05), while the content of cytokine IL-1 尾 decreased significantly (P 0.05). And the secretory immunoglobulin A Siga secretion of duodenum, ileum and cecum was significantly increased (P 0.05). The results showed that the supplementation of 0.025% Emei Xuedan pellet could enhance the immune function of weaned piglets. The effect of Emei Xuedan pellet on serum biochemical indexes of weaned piglets was compared with that of antibiotic group. Adding 0.025% Emei Xuedan pellet to weaning diet could increase the concentrations of total protein (TP), albumin (ALB) and globulin (GLB) in serum of piglets, and the difference was significant in the third week. The concentration of urea nitrogen in serum decreased significantly. The results showed that Emei Xuedan pellet could improve the serum biochemical index of weaning piglets. It can improve the GSH activity of piglets and reduce the content of MDA to some extent. The results showed that Emei Xuedan pellet as a Chinese herbal feed additive could enhance the antioxidant ability of weaned piglets.
【学位授予单位】：四川农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S853.7

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