大豆肽对仔猪免疫和消化功能的影响

发布时间：2018-05-20 04:25

本文选题：大豆肽 + 仔猪　；参考：《江南大学》2016年硕士论文

【摘要】：大豆肽是由大豆分离蛋白经复合酶水解后获得的小肽,其低分子量(相对分子质量主要为150-1500 Da)的特点使其具备很高的应用价值。本文研究了大豆肽对仔猪血液生化指标、肠道组织结构和消化酶活性、免疫功能、氧化还原状态、细胞因子相关基因及先天免疫受体基因表达量等的影响,初步探讨大豆肽的功能及可能的作用机制,从而为更好地开发大豆肽作为新型安全的植物蛋白原料提供一定理论依据。首先以DPPH·和·OH的清除率为指标,对大豆肽在体外的抗氧化活性进行测定。接着选取健康的7日龄“杜×长×大”仔猪12头,分别标记耳号,以雌雄各半的比例分为两组。于每天早上9:00灌胃一次,实验组仔猪灌胃大豆肽溶液250 mg/kg·bw,对照组仔猪灌注丙氨酸溶液254 mg/kg·bw的。实验周期为14天。整个实验期间仔猪由母猪常规哺乳,同时统一饲喂乳猪前期配合饲料,记录初重和末重,试验结束时屠宰取样。根据灌胃实验结果,选取分别标记耳号的、健康的25日龄“杜×长×大”断奶仔猪l44头,以12头猪作为一个重复,三个重复为一组,随机分为4组。分别饲喂含0、5、10、20 g/kg大豆肽的日粮,饲喂周期28天。记录仔猪始末重及饲料消耗量。实验结束时,每个处理组的12头仔猪被随机选取,经前腔静脉采集血样进行检测。实验结果显示:(1)大豆肽对DPPH·和·OH的清除率可分别高达98.33%、84.38%,结果显示大豆肽的抗氧化能力较高,能发挥良好的清除自由基的功效。(2)与对照组相比,大豆肽可提高哺乳仔猪的日增重、血糖浓度,显著降低血浆尿素氮浓度(P0.05);大豆肽显著降低了小肠各段的隐窝深度及绒毛高度与隐窝深度的比值(V/C)(P0.05),小肠胰蛋白酶(P0.05)及二糖酶(P0.01)的活性均显著升高;回肠派伊尔结(PP)的数量显著增加,血浆IgG、IL-6和IFN-γ的浓度也显著提高(P0.01);回肠黏膜sIgA、IL-6和IFN-γ的含量也显著增加;血浆及组织(肝脏、脾脏、十二指肠、空肠、回肠)中SOD的活性得到极显著的提高(P0.01),同时血浆的T-AOC能力也极显著提升(P0.01);经相关性分析显示,血浆T-AOC和SOD活性与IgG的含量呈显著正相关(P0.05);回肠黏膜和肝脏的IFN-γ、IL-6、IL-10、FOXP3和TGFβ1的基因表达均显著上调(P0.05),回肠TLR2、TLR4、TLR9和NF-κB2基因表达量均得到显著上调(P0.05)。(3)添加10 g/kg的大豆肽可以显著提高断奶仔猪的日增重、日采食量(P0.05),料重比也显著降低(P0.05);同时还可以显著提高断奶仔猪的血浆IgG、IgM、IL-6以及IFN-γ含量(P0.05)。综上所述,大豆肽能促进仔猪肠黏膜组织结构发育,提高消化酶活性,促进仔猪对营养的消化吸收,提高肠黏膜免疫功能,改善仔猪生长性能。同时大豆肽有助于提高仔猪抗氧化能力,上调免疫相关基因表达量,从而提高IgG和sIgA含量,增强仔猪免疫功能,更好促进仔猪的健康。
[Abstract]:Soy peptide is a small peptide obtained by hydrolysis of soybean protein isolate after complex enzyme hydrolysis. Its low molecular weight (relative molecular weight is mainly 150-1500 Da) has high application value. In this paper, the blood biochemical index, intestinal structure and digestive enzyme activity, immune function, redox state and cell cause of soybean peptide to piglet were studied. The effects of subrelated genes and the expression of innate immune receptor gene are discussed, and the function and possible mechanism of soybean peptides are preliminarily discussed in order to provide a certain theoretical basis for the better development of soybean peptide as a new safe plant protein raw material. First, the antioxidant activity of soybean peptide in vitro is based on the scavenging rate of DPPH and. OH. Then, 12 healthy 7 days old "Du x long x" piglets were selected to mark the ears, respectively, to be divided into two groups in the proportion of male and male. At 9:00 every morning, the experimental group was intragastric at 9:00. The experimental group was filled with soybean peptide solution of 250 mg/kg. Bw. The control group was perfused with alanine solution of 254 mg/kg. BW. The experiment period was 14 days. The experiment period was the whole experiment period. According to the results of the experiment, the L44 head of a healthy 25 day old Weanling Piglet, "Du x long x big", was selected as a repeat and three repetition as a group and divided into 4 randomly divided into 4 according to the results of the gavage experiment. Group. Fed with 0,5,10,20 g/kg soybean peptide diet, feeding cycle for 28 days. Record the weight and feed consumption of piglets. At the end of the experiment, 12 piglets in each treatment group were randomly selected and collected through the anterior vena cava. The results showed: (1) the scavenging rate of soybean peptide to DPPH and OH could be as high as 98.33%, 84.38%, respectively. The results showed that the antioxidant capacity of soybean peptide was higher and the free radical scavenging effect was good. (2) compared with the control group, soybean peptide could increase the daily weight gain, blood glucose concentration, and significantly lower the plasma urea nitrogen concentration (P0.05), and the soybean peptide significantly reduced the depth of the recess in the small intestine and the ratio of the height of the villi to the depth of the recess. V/C) (P0.05), the activity of trypsin (P0.05) and two glucoenzyme (P0.01) in the small intestine increased significantly, the number of PP in ileum increased significantly, and the concentration of IgG, IL-6 and IFN- y in plasma was also significantly increased (P0.01); the contents of sIgA, IL-6 and IFN- gamma in the ileum mucosa were also significantly increased; plasma and tissues (liver, spleen, duodenum, jejunum, and ileum) were also increased. The activity of the plasma was significantly improved (P0.01), and the T-AOC ability of plasma was significantly increased (P0.01). The correlation analysis showed that the activity of plasma T-AOC and SOD was positively correlated with the content of IgG (P0.05), and the gene expression of IFN- gamma, IL-6, IL-10, FOXP3 and TGF beta 1 in the ileum mucosa and liver were significantly up-regulated. The expression of NF- kappa B2 gene expression was significantly up-regulated (P0.05). (3) adding 10 g/kg soybean peptide could significantly increase the daily weight gain of weaned piglets, daily feed intake (P0.05), and significantly lower feed weight ratio (P0.05). At the same time, the plasma IgG, IgM, IL-6 and IFN- gamma content of weaned piglets were significantly increased (P0.05). To sum up, soybean peptides could promote piglets. The tissue structure of the intestinal mucosa is developed, the digestive enzyme activity is raised, the piglets can improve the digestion and absorption of nutrition, improve the immune function of the intestinal mucosa and improve the growth performance of the piglets. At the same time, the soybean peptide can improve the antioxidant capacity of piglets and raise the expression of the immune related genes, thus improve the content of IgG and sIgA, enhance the immune function of piglets, and promote the piglets better. Health.
【学位授予单位】：江南大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：S828.5

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