和田羊KAPs、KRT候选基因的遗传变异与羊毛细度和长度的相关性分析

发布时间：2018-05-21 15:20

本文选题：和田羊 + 角蛋白基因　；参考：《新疆农业大学》2015年硕士论文

【摘要】：本试验运用PCR-SSCP和DNA测序技术,对和田羊角蛋白家族5个候选基因遗传多态性进行了研究;利用SAS8.1最小二乘方差分析其与羊毛长度和细度的关系,并进一步分析了多基因组合对新疆和田羊羊毛性状的影响。旨在找出与羊毛性状相关基因,为今后和田羊羊毛性状的改善以及育种工作提供科学依据,从而提高牧民的经济收入。KAP6.1基因在碱基序列非编码区内含子发生C51T和G57A两处突变;该基因与羊毛长度及细度关联结果差异均不显著(P0.05)。KAP7基因碱基在序列T110G和G268A处发生突变。AA和AB基因型的羊毛长度差异不显著,AA、AB基因型与BB基因型差异极显著;三个基因型对羊毛细度差异均不显著。KAP7基因可能作为和田羊毛长度性状的一个DNA分子标记。KRT27基因碱基序列中在G49A、T58C两处发生突变;SS基因型与ST、TT基因型比较,羊毛长度及细度差异均显著(0.01P0.05);ST基因型和TT基因型比较,羊毛长度及细度差异均极显著(P0.01)。KRT27基因可能作为和田羊羊毛长度、细度的一个DNA分子标记。KRT31基因碱基序列发生突变C52T。与羊毛长度性状关联分析中,EE基因型和EF、FF基因型差异显著(0.01P0.05),FF基因型极显著长于EF基因型(P0.01);与羊毛细度性状关联分析中,三个基因型差异均不显著(P0.05)。KRT31基因可能作为和田羊羊毛长度的一个DNA分子标记。本试验五个基因中,KAP8基因未检测出多态性;KAP6.1、KAP7、KRT27和KRT31在和田羊群体中突变位点均处于Hardy-Weinberg平衡状态(P0.05)。在新疆和田羊群体中,利用基因聚合技术分析,组合合并基因型优势效应在羊毛细度上未见到体现,而在羊毛长度上表现出了相应的优势。为今后进一步采用多基因聚合育种技术研究和田羊羊毛性状提供了相应的参考依据。
[Abstract]:The genetic polymorphisms of five candidate genes of the Hetian sheep keratin family were studied by using PCR-SSCP and DNA sequencing techniques, and the relationship between the genetic polymorphisms of five candidate genes and wool length and fineness was analyzed by SAS8.1 least square variance analysis. The effects of polygene combinations on wool traits of Xinjiang Hetian sheep were further analyzed. The aim of this study was to find out the genes related to wool traits, and to provide scientific basis for the improvement of wool traits and breeding work in Hetian sheep in the future, so as to increase the economic income of herdsmen. KAP6.1 gene had two mutations in the intron of non-coding region of base sequence, C51T and G57A. There was no significant difference between the length of wool and the length and fineness of wool. There was no significant difference in wool length between AA and AB genotypes in sequence T110G and G268A, and there was no significant difference between AA AB genotype and BB genotype in sequence T110G and G268A. There was no significant difference in wool fineness between three genotypes. KAP7 gene might be used as a DNA molecular marker. KRT27 gene base sequence of the Hetian wool length trait had a mutation of SS genotype and STTTT genotype at two locations of G49AfT58C. There were significant differences in wool length and fineness between the St genotype and TT genotype of 0.01P0.05, and the differences of wool length and fineness were very significant. The gene P0.01U. KRT27 might be used as a DNA molecular marker for wool length of Hotan Sheep. The base sequence of the KRT31 gene was mutated C52T. In the correlation analysis with wool length traits, there were significant differences between EE genotype and EFF FF genotype (0.01P0.05F), which were significantly longer than that of EF genotype (P0.01A), and were correlated with wool fineness traits (P 0.01). The differences among the three genotypes were not significant (P 0.05N. KRT31). KRT31 gene might be used as a DNA molecular marker for wool length of Hetian sheep. In this study, no polymorphic KAP6.1, KAP7, KRT27 and KRT31 mutation loci were found in the Hardy-Weinberg equilibrium state of the five genes in the Hetian sheep population. In Xinjiang Hotan sheep population, the combination genotype dominance effect was not reflected in wool fineness, but in wool length. The results provided a reference basis for the further study of polygene polymerization breeding and wool traits in sheep.
【学位授予单位】：新疆农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S826

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