口蹄疫、猪瘟、高致病性猪繁殖与呼吸综合征三种疫苗同步两点免疫在规模猪场的应用试验

发布时间：2018-05-24 15:49

本文选题：口蹄疫 + 猪瘟　；参考：《中国动物传染病学报》2017年06期

【摘要】：为探索和评估口蹄疫(foot-and-mouth disease,FMD)、猪瘟(classical swine fever,CSF)、高致病性猪繁殖与呼吸综合征(highly pathogenic porcine reproductive and respiratory syndrome,HP-PRRS)的合理免疫程序,选择6个规模猪场,其中3个猪场采用"332法"免疫程序(即先用FMD和CSF疫苗同时在颈部两侧注射,间隔14 d后再用HP-PRRS疫苗注射),另3个猪场采用"321法"免疫程序(即将CSF和HP-PRRS疫苗混合后作1针在颈部一侧注射,FMD疫苗作1针在颈部另一侧注射),每季度采集血清和病死猪组织样品。应用ELISA方法检测血清中的FMD、CSF和HP-PRRS抗体,应用RT-PCR方法检测组织样品中的FMD、CSF和HP-PRRS病原体;统计分析抗体阳性率、发病率、死亡率、死亡猪病原阳性率等评价指标。结果显示,应用"321法"的3个猪场与应用"332法"的3个猪场相比,其生猪死亡率(下降1.3%)、HP-PRRS病原体阳性率(下降6.6%)显著下降(P0.05),而其他考核指标则差异不具备显著统计学意义(P0.05)。本研究表明,规模猪场应用"321法"免疫程序的免疫效果优于"332法"免疫程序的免疫效果,值得在临床推广应用。
[Abstract]:In order to explore and evaluate the rational immunization procedures of foot-and-mouth disease (FMD), classical swine feverfen (CSF) and highly pathogenic porcine reproductive and respiratory syndrome (HP-PRRSv) of foot-and-mouth disease (FMD), six pig farms were selected. Three of the farms were immunized by "332 method" (FMD and CSF vaccines were first injected on both sides of the neck. After 14 days, 3 other pig farms were immunized with the "321 method" (one dose of CSF and HP-PRRS vaccine was injected on one side of the neck) and the other side of the neck was injected with the other side of the neck. The serum was collected quarterly. And tissue samples of dead pigs. FMD-CSF and HP-PRRS antibodies in serum were detected by ELISA method, and FMD-CSF and HP-PRRS pathogens in tissue samples were detected by RT-PCR method. The results showed that compared with 3 farms using "332 method", the mortality rate of live pigs (decreased by 1.3% HP-PRRS pathogen positive rate (down 6.6%) significantly decreased P0.05%, but the difference of other test indexes was not statistically significant (P 0.05%), the results showed that the mortality rate of live pigs was decreased by 1.3HP-PRRS pathogen positive rate (down 6.6%), but the difference of other test indexes was not statistically significant compared with the three farms using "332 method". The results showed that the immune effect of "321 method" was better than that of "332 method", and it was worth popularizing in clinic.
【作者单位】：百色市动物疫病预防控制中心;平果县动物疫病预防控制中心;右江区动物疫病预防控制中心;田东县动物疫病预防控制中心;广西动物疫病预防控制中心;
【基金】：广西科学研究与技术开发计划项目(桂科转14125004-22) 广西水产畜牧科技项目(桂渔牧科201528017) 广西科技重大专项(2017)
【分类号】：S858.28

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