尾分析法在不同规模群体中开展全基因组关联研究

发布时间：2018-05-26 10:14

  本文选题：尾分析法 + 全基因组关联分析　； 参考：《畜牧兽医学报》2017年07期

【摘要】：旨在将尾分析法与全基因组关联分析相结合,初步确定两尾选择的标准。首先,对样本量为2 000、1 500、1 000、500的群体进行全基因组关联分析(GWAS),探索群体规模大小对GWAS结果的影响,然后对各样本量两尾的20%、15%、12%、10%、8%、5%进行GWAS,探索两尾比例的大小对GWAS结果的影响。研究结果表明,检测出的显著关联SNP数目随群体规模及两尾比例的减小而减少。初步确定两尾选择标准为:遗传力为0.38左右的性状,样本量为2 000、1 500、1 000、500,两尾选择比例分别为8%、10%、10%、20%;遗传力为0.50左右的性状,样本量为2 000、1 500、1 000、500,两尾选择比例分别为5%、5%、10%、15%。以此标准为基础进行GWAS,既能有效检测出最显著SNP位点或区间,又能最大程度地降低研究成本,提高研究效率。
[Abstract]:The purpose of this study was to combine tail analysis with genomic association analysis to determine the criteria for selection of two tails. Firstly, the whole genome association analysis was carried out on a population with a sample size of 2 000 / 1 500 / 1 000500 to explore the effect of population size on the results of GWAS, and then GWASS was carried out on 20 / 15 / 12 / 1088% of the two samples to explore the effect of the size of the two tails on the results of GWAS. The results show that the number of significant association SNP detected decreases with the decrease of population size and two-tail ratio. The selection criteria of two tails were as follows: the heritability was about 0.38, the sample size was 2 000 / 1 500, the ratio of the two selection was 8 / 10 / 10 / 10 / 20, the sample size of the traits with heritability of about 0.50 was 2 000 / 1 500 / 100 / 500, and the ratio of the two selections was 5 / 10 / 10 / 1 / 500 and the ratio of the two selections was 5 / 10 / 10 / 15 / 500, respectively, in which the heritability was about 0. 50 and the sample size was 2 000 / 1 / 100 / 100 / 500, respectively. On the basis of this standard, GWASs can be used to detect the most significant SNP loci or regions, reduce the research cost and improve the research efficiency.
【作者单位】： 湖南农业大学动物科学技术学院;中国农业科学院北京畜牧兽医研究所;湖南省畜禽安全生产协同创新中心;
【基金】：国家科技支撑计划项目(2015BAD03B02-2) 中国农业科学院科技创新工程(ASTIP-IAS02) 国家生猪产业技术体系(CARS-36)
【分类号】：S813.1
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本文编号：1936853