不同鸡品种BNK基因序列比较分析

发布时间：2018-05-26 14:11

  本文选题：鸡 + BNK基因　； 参考：《浙江农业学报》2017年10期

【摘要】：BNK(C-type lectin-like receptor 2)基因为C型凝结素受体基因中的抑制性基因,与禽类先天免疫性能关系密切。本研究以NCBI中Gallus gallus BNK基因序列为参考,利用MEGA6软件对24个鸡品种的BNK基因进行序列比较分析,筛选出外显子和内含子上的SNPs位点、Indels以及氨基酸变化,并对24个鸡品种进行系统聚类。研究发现,SNPs位点大多发生在Exon3、Exon5和Exon6上,且多为错义突变。内含子上的SNPs位点大多发生在Intron1、Intron2和Intron4上。插入缺失发生在Intron2、Intron4和Exon5上,并且大多数品种Indels序列基本一致,国内外鸡品种之间并无显著差异。系统聚类分析将这些鸡品种分为3类,狼山鸡、罗斯鸡、隐性白羽肉鸡、茶花鸡和如皋黄鸡为一类;其次,雪山鸡、安卡鸡、斗鸡和萧山鸡聚为一类;固始鸡单独为一类,表明固始鸡的BNK基因为单独起源。综上表明,BNK基因在我国地方鸡品种中具有丰富的遗传多样性,与不同鸡品种的先天免疫性能存在关联,这为家禽的免疫遗传学和抗病育种提供了参考依据。
[Abstract]:BNK(C-type lectin-like receptor 2) gene is an inhibitory gene of type C lectin receptor gene, which is closely related to the innate immunity of poultry. In this study, the sequence of Gallus gallus BNK gene in NCBI was compared and analyzed by MEGA6 software, and the SNPs loci on exon and intron were screened out. Systematic clustering was carried out on 24 chicken breeds. It was found that most of the SNPs loci occurred on Exon3 exon5 and Exon6, and most of them were missense mutations. Most of the SNPs loci in intron are on Intron1, Intron2 and Intron4. The insertion deletion occurred on Intron2, Intron4 and Exon5, and the Indels sequence of most breeds was basically the same, but there was no significant difference between domestic and foreign chicken breeds. According to the systematic cluster analysis, these chicken breeds were divided into three categories: Wolfhill chicken, Roth chicken, recessive white feather broiler, camellia chicken and Rugao yellow chicken; secondly, Xueshan chicken, Anka chicken, fighting chicken and Xiaoshan chicken were grouped into three categories; The results showed that the BNK base of Gushi chicken was isolated. It is concluded that the BNK gene has abundant genetic diversity in local chicken breeds in China, which is related to the innate immune performance of different chicken breeds, which provides a reference for the breeding of poultry immune genetics and disease resistance.
【作者单位】： 扬州大学动物科学与技术学院;
【基金】：国家自然科学基金(31372297) 江苏省六大人才高峰项目(2015-NY-019)
【分类号】：S831.2
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本文编号：1937517