高浓度皮质醇对大肠杆菌诱导的山羊急性子宫内膜炎的作用研究

发布时间：2018-05-26 14:57

本文选题：子宫内膜炎 + 大肠杆菌　；参考：《扬州大学》2017年硕士论文

【摘要】：子宫内膜炎是一种主要影响产乳动物的产后疾病,通常由病原微生物感染引起。该疾病可引起产奶量下降,妊娠失败率增加,造成重大的经济损失。皮质醇是一种重要的炎症调节物质,其在炎症损伤中具有重要作用。在分娩过程中,母体血液中皮质醇水平升高。本实验重点观察,高浓度的皮质醇对山羊大肠杆菌性子宫内膜炎的调节作用机制。12只健康的山羊随机分为三组:对照组、大肠杆菌组和皮质醇治疗组。皮质醇治疗组,每天按1mg/kg的剂量每天静脉滴注皮质醇,3天后,子宫灌注大肠杆菌;大肠杆菌组每天静脉注射生理盐水,3天后,子宫灌注大肠杆菌;对照组每天静脉注射生理盐水,3天后,子宫灌注PBS。接种E.coli后0、12、24、96和168h,进行白细胞计数、体温、脉搏和呼吸的检测;接种E.coli后0、24、96和168h,进行血液皮质醇含量检测。收集子宫分泌物进行细胞学检查及细菌培养;采用HE染色法对子宫内膜的组织进行病理学观察;实时荧光定量PCR法检测子宫内膜组织中TLR4、IL-1β、IL-6、IL-8、TNF-α、TGF-β和VEGF。大肠杆菌组和皮质醇治疗组的白细胞数量在12h时与对照组相比显著增加(p0.05),感染24h后,恢复正常。大肠杆菌组和皮质醇治疗组在感染12h后体温升高。皮质醇治疗组血液皮质醇含量在每个时间点均高于空白组与大肠杆菌组。皮质醇治疗组感染大肠杆菌24h时,呼吸频率急剧上升,但在感染96h时恢复正常。三个组之间的心率没有明显变化。大肠杆菌组和皮质醇治疗组的子宫分泌物在接种24h时可见到大量的嗜中性粒细胞。大肠杆菌组及皮质醇治疗组子宫内膜组织中的白细胞数量在接种24h时与对照组相比升高,之后逐渐减少。感染12和24h时,大肠杆菌及皮质醇治疗组子宫分泌物内获得阳性细菌。大肠杆菌组和皮质醇治疗组子宫内膜组织中的TLR4、IL-1β和TNF-α的mRNA表达量在感染24h时与对照组相比没有显著差异,但皮质醇治疗组的IL-6和IL-8水平在感染后24h和96h时显著降低(p0.05)。在感染前后,大肠杆菌组和皮质醇治疗组子宫内膜组织中的TGF-β mRNA表达与对照组相比差异不显著。皮质醇治疗组的VEGF mRNA表达量在感染24h后与大肠杆菌组相比显著降低(p0.01)。
[Abstract]:Endometritis is a postpartum disease that mainly affects lactating animals and is usually caused by pathogenic microbial infections. The disease can lead to a decline in milk production and an increase in pregnancy failure rates, resulting in significant economic losses. Cortisol is an important inflammatory regulator, which plays an important role in inflammatory injury. During delivery, cortisol levels in maternal blood are elevated. In this study, we observed the regulatory mechanism of high concentration cortisol on Escherichia coli endometritis. Twelve healthy goats were randomly divided into three groups: control group, Escherichia coli group and cortisol treatment group. In the cortisol treatment group, colibacillosis was infused into the uterus 3 days after intravenously infusion of cortisol at the dose of 1mg/kg, while in the Escherichia coli group, E. coli was infused into the uterus after 3 days of intravenous injection of normal saline. In the control group, PBSs were perfused into uterus 3 days after intravenously injection of normal saline. The white blood cell count, body temperature, pulse and respiration were detected at 0 h after inoculation with E.coli, and the contents of cortisol were detected at 0 h, 24 h and 168 h after E.coli inoculation. The samples were collected for cytological examination and bacterial culture, the histopathology of endometrium was observed by HE staining, and the levels of TLR4, IL-1 尾, IL-6, IL-8, TNF- 伪 TGF- 尾 and VEGF in endometrial tissues were detected by real-time fluorescence quantitative PCR. Compared with the control group, the number of white blood cells in Escherichia coli group and cortisol treatment group increased significantly at 12 h, and returned to normal 24 hours after infection. The body temperature of Escherichia coli group and cortisol treatment group increased 12 hours after infection. The blood cortisol content in cortisol treatment group was higher than that in blank group and Escherichia coli group at each time point. In the cortisol treated group, the respiratory rate increased sharply at 24 h, but returned to normal at 96 h. There was no significant change in heart rate between the three groups. A large number of neutrophils were found in uterine secretions of Escherichia coli and cortisol treatment groups after 24 h inoculation. The number of leukocytes in endometrial tissue of Escherichia coli group and cortisol treatment group increased after 24 h inoculation compared with control group, and then decreased gradually. Positive bacteria were obtained in uterine secretions of Escherichia coli and cortisol treatment group at 12 and 24 hours of infection. There was no significant difference in the mRNA expression of TLR4, IL-1 尾 and TNF- 伪 in endometrium of Escherichia coli group and cortisol treatment group, but the levels of IL-6 and IL-8 in cortisol treated group were significantly lower than those in control group at 24 h and 96 h after infection. Before and after infection, the expression of TGF- 尾 mRNA in endometrium of Escherichia coli group and cortisol group was not significantly different from that of control group. The expression of VEGF mRNA in the cortisol treated group was significantly lower than that in the Escherichia coli group 24 hours after infection.
【学位授予单位】：扬州大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S858.27

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