乌珠穆沁羊Myostatin基因序列分析及多态性研究

发布时间：2018-05-26 21:25

本文选题：乌珠穆沁羊 + Myostatin　；参考：《内蒙古农业大学》2015年硕士论文

【摘要】：本研究采用自然放牧条件下(多胸椎个体)乌珠穆沁羊,从扩增乌珠穆沁羊Myostatin (MSTN)基因全序列入手,对该品种MSTN基因的序列结构、核苷酸组成进行了分析,并对其编码蛋白质进行了预测分析,构建该基因分子进化树；同时分析了乌珠穆沁羊MSTN基因部分序列的多态性。结果显示：MSTN基因由3个外显子和2个内含子组成,其外显子大小分别为373bp、374bp、381bp,内含子大小分别为1833bp、2030bp。CDS序列全长为1128bp,由375个氨基酸组成。利用生物信息学软件分析发现：乌珠穆沁羊MSTN基因编码蛋白的理论分子量约为42.8kDa, PI值为7.01,Leu的含量最高(9.9%),其次是Lys(8.3%)。乌珠穆沁羊基因编码蛋白二级结构以随机卷曲结构为主,其蛋白属于跨膜蛋白,AA6-AA23之间为跨膜区,具有一个分泌信号肽结构。其氨基酸序列MQKLQICVYIYLFMLIVA具有TGF-β家族的特征。P6引物扩增的366bp的区域,在位于CDS序列第126bp处发生C→T的沉默突变,表现EE和EF两种基因型。引物P7扩增的353bp的区域,在所检测的范围内没有发现多态性。P8引物扩增的349bp的区域,在位于CDS序列第909bp处有一个A→G的沉默突变,表现AA和AB两种基因型。本研究为改善地方优良品种以及利用该基因开展乌珠穆沁羊分子育种技术研究等提供理论基础,并为建立乌珠穆沁羊基因文库提供可靠信息。
[Abstract]:In this study, the sequence structure and nucleotide composition of Myostatin MSTN gene were analyzed by amplifying the whole sequence of Myostatin MSTN gene under natural grazing condition (individual multiple thoracic vertebrae). The coding protein was predicted and analyzed to construct the molecular evolutionary tree of the gene, and the polymorphism of some MSTN gene sequences of Wudhumuqin sheep was also analyzed. The results showed that: MSTN gene consists of three exons and two introns, the exon size is 373bpn374bpP3381bprespectively, and the intron size is 1833bp 2030bp.CDS sequence is 1128bp, which consists of 375 amino acids. The analysis of bioinformatics software showed that the theoretical molecular weight of the protein encoded by MSTN gene was about 42.8 kDa, and the Pi value of 7.01% Leu was the highest (9.9%), followed by Lys-8.3kDa. The secondary structure of the protein encoded by Uzhimuqin sheep gene is mainly random curl structure. The protein belongs to the transmembrane region between AA6-AA23 and has a secretory signal peptide structure. The amino acid sequence MQKLQICVYIYLFMLIVA had the characteristic of TGF- 尾 family. P6 primer amplified the region of 366bp. The silencing mutation of C T occurred at the 126bp of CDS sequence, showing two genotypes EE and EF. In the region of 353bp amplified by primer P7, there was no polymorphic region of 349bp amplified by P8 primer. There was a silencing mutation of A G at the 909bp of CDS sequence, showing two genotypes AA and AB. This study provides a theoretical basis for the improvement of local fine varieties and the study of molecular breeding techniques using the gene, and provides reliable information for the establishment of a gene library of Wuzhimuqin sheep.
【学位授予单位】：内蒙古农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S826;Q78

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