治疗奶牛子宫内膜炎中药复方泡腾栓的研究

发布时间：2018-05-26 22:01

本文选题：奶牛子宫内膜炎 + 中药　；参考：《东北农业大学》2015年硕士论文

【摘要】：奶牛子宫内膜炎是造成奶牛不孕的主要原因之一,对奶牛养殖业有着严重的危害,研究出治疗该病的良方迫在眉睫。应用中药组方不仅能够保证疗效,还能减小药物残留,改善菌株耐药性的问题。本研究根据中兽医理论选择多种中草药,采用正交试验,对奶牛子宫内膜炎的4种主要致病菌进行体外抑菌试验,根据中草药体外抑菌结果,筛选由双花、黄芩、等8味中草药,组合而成得到抑菌作用比较明显的复方。泡腾栓具有刺激性小、使用方便,在与体液接触后可产生大量泡沫,能够深入子宫黏膜的深处,将药物释放到细菌的感染之处,最大限度的发挥药效。本试验用正交法对泡腾栓基质进行筛选,以筛选的中药组方为主药,制备中药泡腾栓。对制备的泡腾栓的外观、重量差异、融变时限、熔点范围、变形温度、p H等理化性状进行了检查和测定;用中药泡腾栓对4种致病菌进行了体外抑菌试验。结果表明,中药泡腾栓的最佳配比为:PEG-6000 1.4g,碳酸氢钠0.26g,柠檬酸0.24g,吐温-80 0.03g,主药0.404g;中药泡腾栓的理化性状均符合中国药典(2010版)规定;中药泡腾栓对4种致病菌的抑制效果与原药相近。采用高效液相色谱(HPLC)法对中药泡腾栓中的几种单味中药的有效成分绿原酸和黄芩苷进行了含量测定,考察了产品质量的稳定性和可控性。绿原酸和黄芩苷分别在3.2-25.6μg/mL和20.8-62.4μg/mL范围内有着良好的线性关系;二者的精密度和重现性均为良好;绿原酸和黄芩苷在每粒中药泡腾栓中的含量分别为0.00291g和0.01348g;绿原酸和黄芩苷的平均加样回收率分别为99.46%和99.23%。结果表明,该方法对于质量控制是稳定、可控的。
[Abstract]:Endometritis is one of the main causes of infertility in dairy cows. The application of traditional Chinese medicine can not only guarantee the curative effect, but also reduce the drug residue and improve the drug resistance of strains. In this study, according to the theory of Chinese veterinarian, many kinds of Chinese herbal medicines were selected, and four main pathogenic bacteria of endometritis of dairy cows were tested by orthogonal test in vitro. According to the results of bacteriostasis of Chinese herbal medicine in vitro, Scutellaria baicalensis were selected from Shuanghua and Scutellaria. 8 Chinese herbal medicines, combined to obtain a more obvious bacteriostatic effect of compound. Effervescent suppository has small irritation, easy to use, can produce a large number of foam after contact with body fluid, can go deep into the uterine mucosa, release the drug to the bacterial infection, and give full play to the drug effect. In this experiment, the matrix of effervescent suppository was screened by orthogonal method, and the Chinese medicine effervescent suppository was prepared. The physical and chemical properties of the effervescent suppository, such as appearance, weight difference, melting time, melting point range, deformation temperature and pH, were examined and determined, and four pathogenic bacteria were tested in vitro with Chinese medicine effervescent suppository. The results showed that the optimum proportion of Chinese medicine effervescent suppository was: 1: PEG-6000, sodium bicarbonate 0.26g, citric acid 0.24g, Tween -800.03g, main drug 0.404g.The physicochemical properties of Chinese medicine effervescent suppository were in accordance with the Chinese Pharmacopoeia 2010 edition. The inhibitory effect of traditional Chinese medicine effervescent suppository on 4 kinds of pathogenic bacteria was similar to that of original medicine. High performance liquid chromatography (HPLC) was used to determine the contents of Lv Yuan acid and baicalin in several kinds of Chinese medicine effervescent suppositories. The stability and controllability of the products were investigated. The linear range of Lv Yuan acid and baicalin was 3.2-25.6 渭 g/mL and 20.8-62.4 渭 g/mL, respectively. The contents of Lv Yuan acid and baicalin in each Chinese medicine effervescent suppository were 0.00291g and 0.01348g, respectively, and the average recoveries of Lv Yuan acid and baicalin were 99.46% and 99.23g, respectively. The results show that the method is stable and controllable for quality control.
【学位授予单位】：东北农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S858.23

【参考文献】