不同剂量6-羟多巴胺对大鼠子宫交感神经损毁情况及其对肥大细胞分布的影响

发布时间：2018-05-27 07:18

本文选题：大鼠 + 子宫　；参考：《中国兽医学报》2016年12期

【摘要】：为研究6-羟多巴胺(6-OHDA)损毁大鼠子宫交感神经的剂量及其对子宫肥大细胞分布的影响,选取180~200日龄健康的性成熟Wistar雌性大鼠24只,随机分为对照组和处理组,处理组尾静脉注射25,50,100 mg/kg的6-OHDA。结果显示:按照一次性注射100mg/kg 6-OHDA的方法可以损毁支配子宫的交感神经,而50 mg/kg未能损毁子宫内膜固有层的神经。肥大细胞观察结果显示,其主要分布在内环、外纵肌之间的血管周围,随着6-OHDA剂量的不断加大,肥大细胞数量呈减少趋势,3个处理组对比差别显著,对照组略高于25 mg/kg组。血清中组织胺含量呈相反的变化趋势。结果表明,可采取一次性注射100mg/kg的6-OHDA来建立交感神经损毁模型,交感神经影响子宫内肥大细胞的分布,对于机体组织胺释放的调控不同于子宫。
[Abstract]:In order to study the dose of 6-hydroxydopamine 6-OHDA-damaged uterine sympathetic nerve and its effect on the distribution of uterine mast cells, 24 healthy female sexual mature Wistar rats aged 180 ~ 200 days were randomly divided into two groups: control group and treatment group. In the treatment group, 6-OHDA-25 mg/kg was injected intravenously into the caudal vein. The results showed that the sympathetic nerves innervated the uterus could be damaged by a single injection of 100mg/kg 6-OHDA, while the nerve in the lamina propria of the endometrium could not be damaged by 50 mg/kg. The results of mast cell observation showed that the mast cells were mainly distributed in the inner ring and around the blood vessels between the external longitudinal muscles. With the increasing of 6-OHDA dose, the number of mast cells decreased. The differences among the three treatment groups were significant, and the control group was slightly higher than 25 mg/kg group. The content of histamine in serum showed a reverse trend. The results showed that the model of sympathetic nerve lesion could be established by injecting 6-OHDA of 100mg/kg at one time. Sympathetic nerve affected the distribution of mast cells in uterus and the regulation of histamine release was different from that of uterus.
【作者单位】：山东农业大学生命科学院;山东农业大学动物科技学院;
【基金】：山东省自然科学基金资助项目(ZR2010CM052) 山东省教育厅高等学校科技计划资助项目(J11LC26)
【分类号】：S859.7

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