40周龄三黄种鸡爆发禽白血病的诊断与病原分析

发布时间：2018-05-28 00:27

本文选题：周龄三黄鸡 + J亚群禽白血病病毒　；参考：《黑龙江畜牧兽医》2017年16期

【摘要】：为了解鸡群中禽白血病(Avian leukosis,AL)发生的原因以及感染的禽白血病病毒(Avian leukosis virus,ALV)的亚群,研究对发病鸡进行临床诊断、病理剖检和病理组织学观察,并采集肿瘤样病变组织处理后接种DF-1细胞,培养后分别进行禽白血病病毒p27群特异性抗原ELISA检测、亚群特异性PCR检测以及亚群特异性间接免疫荧光法(IFA)鉴定,确定了40周龄三黄种鸡群感染了J亚群禽白血病病毒(ALV-J,分离株命名为GX16YL02);进一步对分离株env基因进行PCR扩增、克隆和序列测定与比较分析。结果表明:分离株GX16YL02的env基因与ALV-J英国原型株HPRS-103的核苷酸及氨基酸的相似性分别为93.9%和91.9%,与其他8株国内外参考株的核苷酸及氨基酸的相似性分别为90.7%~95.5%和89.4%~95.2%,其中gp85基因与其他参考株的核苷酸和氨基酸相似性相对较低,分别为86.9%~94.9%和85.1%~95.4%,整个env基因有义突变和沉默突变的比例分析显示,与gp37基因相比,gp85基因变异较大,尤其是免疫选择压在高变区hr1区域有一定的作用。
[Abstract]:In order to understand the causes of avian leukemia (Avian leukosissis) and the subsets of avian leukemia virus (Avian leukosis virus) in chickens, the clinical diagnosis, histopathology and histopathology of the infected chickens were studied. DF-1 cells were collected and inoculated with tumor-like lesion tissues, and then detected by ELISA, subgroup specific PCR and indirect immunofluorescence assay of avian leukemia virus p27 specific antigen, respectively. Avian Leukemia virus subgroup J ALV-J was infected in 40 week-old Sanhuang chickens, and the isolated strain was named GX16YL02.The env gene of the isolate was amplified by PCR, cloned, sequenced and compared. The results showed that the similarity of nucleotide and amino acid between env gene of GX16YL02 and HPRS-103 of ALV-J was 93.9% and 91.9%, respectively. The similarity of nucleotide and amino acid of env gene with other 8 reference strains was 90.795% and 89.495%, respectively. The nucleotide and amino acid similarity of gp85 gene was relatively low with other reference strains. 86.9% and 85.1%, respectively. The proportion of sense mutation and silencing mutation in the whole env gene was 86.9% and 85.1%, respectively. The results showed that the mutation of gp85 gene was greater than that of gp37 gene, especially the immuno-selective pressure had a certain effect on the hr1 region in the hypervariable region.
【作者单位】：广西大学养禽与禽病学研究所;
【基金】：国家现代农业产业技术体系广西肉鸡产业创新团队建设项目(nycytxgxcxtd-04-20-2) 广西研究生教育创新计划项目(T3340098003)
【分类号】：S858.31

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