玉米赤霉烯酮对小鼠T淋巴细胞体外活化、增殖的影响

发布时间：2018-05-28 06:28

本文选题：玉米赤霉烯酮 + T淋巴细胞增殖　；参考：《畜牧兽医学报》2017年07期

【摘要】：为揭示镰刀菌毒素之一的玉米赤霉烯酮(ZEA)的免疫抑制作用机制,作者拟研究ZEA对刀豆蛋白A(Con A)介导的小鼠离体T淋巴细胞体外活化、增殖的影响。以Con A作为T淋巴细胞活化刺激剂,以不同浓度ZEA(0、10、20、40μmol·L~(-1))染毒处理细胞后,利用流式细胞术检测T淋巴细胞早期活化标志CD69分子及中期活化标志CD25分子表达情况,用CCK-8法检测T淋巴细胞的增殖情况。结果显示:细胞分别培养48、72、96h后,Con A组与细胞空白组相比均产生了明显的刺激增殖效应(P0.01)。与Con A对照组相比,ZEA染毒可明显抑制Con A刺激引起的T淋巴细胞的增殖作用。除10μmol·L~(-1) ZEA组在72和96h时间段差异显著外(P0.05),其余染毒组在各时间段均差异极显著(P0.01),并呈浓度-效应关系。细胞加入Con A分别活化6、30h后,Con A组与细胞空白组相比,早期活化分子CD69和中期活化分子CD25明显升高,提示细胞发生明显的活化效应。与Con A对照组相比,当ZEA染毒浓度为10μmol·L~(-1)时,T细胞早期活化标志分子CD69和中期活化分子CD25的表达明显抑制,差异均极显著(P0.01),当ZEA浓度为20、40μmol·L~(-1)时,CD69和CD25的表达进一步抑制(P0.01),并呈剂量依赖性。研究结果表明,ZEA对动物免疫抑制作用的产生与其可直接抑制对小鼠T淋巴细胞的活化和增殖有关。
[Abstract]:In order to reveal the immunosuppressive mechanism of ZEA, one of Fusarium toxin, the author intends to study the effect of ZEA on the activation and proliferation of mouse T lymphocytes mediated by concanavalin A(Con A in vitro. Con A was used as the activation stimulator of T lymphocytes. After treated with different concentrations of ZEAA, the expression of CD69 molecule, the early activation marker of T lymphocytes, and the intermediate activation marker, CD25, were detected by flow cytometry. The proliferation of T lymphocytes was detected by CCK-8 method. The results showed that the proliferation effect of Con A group was significantly higher than that of control group (P 0.01). Compared with Con A control group, ZEA significantly inhibited the proliferation of T lymphocytes stimulated by Con A. With the exception of 10 渭 mol / L ~ (-1) ZEA group, there was a significant difference between the two groups in 72 h and 96 h, while the other groups were significantly different in each time period (P 0.01) and showed a concentration-effect relationship. Compared with the control group, the early activated molecule CD69 and the metaphase activated molecule CD25 in the Con A group were significantly higher than those in the control group after the activation of Con A for 6h and 30h, respectively, indicating that the activation effect of Con A group was obvious. When the concentration of ZEA was 10 渭 mol / L ~ (-1), the expression of CD69 and CD25 in T cells was significantly inhibited compared with the control group of Con A. When the concentration of ZEA was 20 ~ 40 渭 mol / L ~ (-1), the expression of CD69 and CD25 was further inhibited in a dose-dependent manner. The results showed that the immunosuppressive effect of ZEA was related to its direct inhibition on the activation and proliferation of T lymphocytes in mice.
【作者单位】：扬州大学兽医学院;江苏省动物重要疫病与人兽共患病防控协同创新中心;
【基金】：国家重点研发计划支持项目(2016YFD0501208) 江苏省普通高校自然科学基金(08KJD230002) 江苏高校优势学科建设工程资助项目(PAPD)
【分类号】：S859.87

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