同胞陪伴对慢性不可预见性温和应激诱导的情绪障碍的缓冲效应及其神经机制研究

发布时间：2018-05-28 20:30

本文选题：棕色田鼠 + 小鼠　；参考：《陕西师范大学》2015年硕士论文

【摘要】：动物生存的社会环境对其生理和行为发育具有深远的影响。社会环境对焦虑抑郁有明显的作用,而社会支持作为一种积极地社会环境被广泛应用于情绪障碍的干预治疗中,但社会环境影响动物焦虑抑郁的神经内分泌机制尚不清楚。同时,传统的实验动物如小鼠和大鼠等不具备复杂的社会行为,因此对社会支持与焦虑抑郁之间的关系及其内在机制的研究难度较大,而社会支持等复杂社会行为却在单配制田鼠中普遍存在,所以单配制田鼠成为社会支持神经生物学研究的重要动物模型。棕色田鼠是一种高社会性的单配制动物,早期生存的社会环境对其成年后情绪及社会性都有影响,这为本研究提供了重要的动物模型。此外,棕色田鼠作为一种野生鼠,其生活习性、社会结构、行为表达等均与经典的实验动物有较大的区别,棕色田鼠对其生存的物理及社会环境进行短期或长期适应,使其对应激的反应可能与小鼠和大鼠也存在一定差异。基于以上研究基础和假设,本研究分别以小鼠和棕色田鼠作为研究对象进行比较研究,通过慢性不可预见性温和应激(Chronic Unpredictable Mild Stress, CUMS)诱导动物情绪障碍,建立应激诱导情绪障碍的模型,在该模型的基础上探究同胞陪伴这种社会支持在CUMS影响动物生理及情绪发育过程中发挥的缓冲效应及其神经机制。主要研究结果如下：1)对雄性小鼠亚成体体重增长率进行研究。结果发现,CUMS能够抑制动物体重的增长,而在群养条件下,同胞陪伴作为一种社会支持能够有效缓冲CUMS引起的对体重增长的抑制作用。2)利用旷场实验和高架十字迷宫实验对动物焦虑水平进行评价。结果发现,饲养模式和／或CUMS均不会改变昆明小鼠亚成体的运动性,但CUMS能够导致单养个体出现焦虑倾向,而同胞陪伴能够缓冲CUMS该效应的发挥。3)利用强迫游泳和糖水偏好对动物抑郁水平进行检测。结果发现,CUMS能够有效诱导其抑郁样行为的表达,而CUMS的这一效应可被同胞陪伴缓冲。4)对各组雄性亚成体昆明小鼠血清糖皮质激素(Glucocorticoid, CORT)含量进行检测。结果发现,同胞陪伴能够提高动物对CUMS的敏感性。5)通过免疫组织化学的方法检测各组雄性小鼠亚成体中脑腹侧被盖区(VTA)酪氨酸羟化酶(Tyrosine Hydroxylase, TH)及海马CA1区糖皮质激素受体(Glucocorticorid Receptor, GR)及下丘脑室旁核(PVN)和视上核(SON)催产素(Oxytocin, OT)神经元的表达。结果发现,VTA的TH虽然与社会支持相关,却并不参与CUMS诱导情绪变化的过程,而海马CA1区GR及PVN和SON的OT神经元则参与同胞陪伴缓冲CUMS诱导情绪变化的过程。6)对棕色田鼠亚成体体重增长率进行比较。结果发现,只有在无应激情况下,同胞陪伴才能够提高雌性的体重增长率,而在接受CUMS情况下,有无同胞陪伴,雌性棕色田鼠体重增长率都较低,说明无同胞陪伴,即单养(社会应激)或CUMS单独作用,均能使其对雌性棕色田鼠亚成体体重增长的抑制作用达到天花板效应。而对于雄性棕色田鼠亚成体,只有既无社会支持又接受CUMS才能显著抑制其体重增长。7)利用旷场实验和高架十字迷宫实验对动物焦虑水平进行评价。结果发现,CUMS能够导致雌雄棕色田鼠亚成体产生焦虑情绪,而同胞陪伴能够缓冲CUMS该效应的发挥。8)利用强迫游泳和糖水偏好对动物抑郁水平进行检测。结果发现,CUMS能够诱导雄性棕色田鼠抑郁样行为的表达,CUMS的致抑郁效应可被同胞陪伴所缓冲。CUMS对雌性棕色田鼠亚成体则无明显影响。综上所述,本研究结果提示小鼠与棕色田鼠对CUMS的反应存在种间差异,棕色田鼠对CUMS的反应也存在明显的性别差异,同胞陪伴作为一种社会支持对应激诱导的焦虑或／和抑郁具有缓冲效应,从而降低应激对情绪造成的负向影响,OT、多巴胺(Dopamine, DA)及CORT系统在此过程中具有调节作用。本研究在一定程度上揭示了社会支持改善焦虑抑郁的规律,阐明了社会环境调节焦虑抑郁的神经内分泌机制,并为进一步对改善动物情绪和福利的研究提供了新思路,为优化动物的饲养与管理奠定了一定的理论基础。
[Abstract]:The social environment of animal survival has a profound influence on its physiological and behavioral development. The social environment has an obvious effect on anxiety and depression, and social support is widely used in the intervention treatment of emotional disorders as a positive social environment. However, the neuroendocrine mechanism of the social environment affecting the anxiety and depression of animals is not yet clear. The traditional experimental animals, such as mice and rats, do not have complex social behavior, so it is difficult to study the relationship between social support and anxiety and depression, and the complex social behavior such as social support is common in the single field mice. So the single field mouse has become a social support neurobiology research. An important animal model. The brown field mouse is a highly socially single animal. The early survival of the social environment has an influence on the emotion and sociality of the adult. This provides an important animal model for this study. In addition, the brown vole, as a wild rat, is a kind of wild rat, and its living habits, social structure, behavior expression and so on are all with the classic reality. There is a big difference between animals and mice. Brown field mice have a short or long term adaptation to their physical and social environment, and the response to stress may be different from that of mice and rats. Based on the above research basis and hypothesis, the mice and brown voles were compared and studied by chronic not. Predictability and mild stress (Chronic Unpredictable Mild Stress, CUMS) induced emotional disorder in animals and the establishment of a model of stress induced emotional disorder. On the basis of this model, the buffering effect and the neural mechanism of the social support of compatriots in the process of CUMS affecting animal physiological and emotional development are explored. The results are as follows: 1) the growth rate of adult male subadult body weight was studied. The results showed that CUMS could inhibit the growth of animal body weight, and under the condition of group breeding, SIB accompany as a social support could effectively buffer the inhibitory effect of CUMS on weight growth,.2) using open field experiment and elevated cross maze test for animal anxiety water. The results showed that both the feeding pattern and / or CUMS did not change the motility of the subadult of Kunming mice, but CUMS could lead to the anxiety tendency of individual individuals, and the siblings could buffer the CUMS effect of.3) using forced swimming and sugar water preference to detect the level of depression in animals. The results found that CUMS could be used. The expression of depression like behavior was induced, and the effect of CUMS could be accompanied by sib buffering.4) to detect the content of serum glucocorticoid (Glucocorticoid, CORT) in the serum of male subadult Kunming mice. The results showed that siblings could improve the sensitivity of animal to CUMS.5) by immunohistochemical method to detect each group of males. The expression of tyrosine hydroxylase (Tyrosine Hydroxylase, TH) in the ventral ventral tegmental area (VTA) and the expression of Tyrosine Hydroxylase (GR) and the hypothalamus paraventricular nucleus (PVN) and the supraventricular nucleus (SON) of the supraventricular nucleus (Oxytocin, OT) of the hypothalamus of the middle cerebral ventral tegmental region of the sexual subadult mice Participation in the process of CUMS induced emotional changes, while the GR and PVN and SON OT neurons in the hippocampus of the hippocampal CA1 were involved in the process of sibling buffer CUMS induced mood changes.) the growth rate of the subadult body weight of the brown voles was compared. In the case of CUMS, with or without SIB, the female brown vole had a lower body weight growth rate, indicating that no SIB, that is, single breeding (social stress) or individual action, could make the female brown field mouse subadult weight increase to reach ceiling effect. For male Brown vole subadult, only neither social support nor connection. CUMS could significantly inhibit its weight gain of.7) using open field experiments and elevated cross maze tests to evaluate the level of animal anxiety. The results showed that CUMS could lead to anxiety in the adult male and female adult Brown vole subadult, while siblings could buffer the CUMS effect of.8) using forced swimming and sugar water preference for animal depression. The results showed that CUMS could induce the expression of depressive behavior in male Brown voles, and the depressive effect of CUMS was not significantly affected by the buffered.CUMS on the female brown field mouse subadult. In summary, the results suggested that there was a difference between the mice and the brown voles' response to the CUMS. There is also a significant gender difference in the response of CUMS. As a social support, compatriot as a social support has a buffer effect on induced anxiety or / and depression, thus reducing the negative impact of stress on emotion. OT, Dopamine, DA and CORT systems have a regulatory role in this process. This study reveals to some extent the society. It will support the law of improving anxiety and depression, clarifies the neuroendocrine mechanism of regulating anxiety and depression in the social environment, and provides a new idea for further research on improving animal mood and welfare, and lays a theoretical foundation for optimizing animal husbandry and management.
【学位授予单位】：陕西师范大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S852.3

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