重组酶聚合酶扩增技术及其在动物病原快速检测中的应用

发布时间：2018-05-29 10:09

本文选题：重组酶聚合酶扩增(RPA) + 检测　；参考：《中国兽医学报》2016年10期

【摘要】：建立动物疫病的快速诊断,对于我国养殖业疫病的预防及控制、进出口检疫、动物食品安全卫生等方面具有重大意义。重组酶聚合酶扩增技术(RPA)是近几年出现的1种有望替代PCR的新的核酸扩增技术。该技术主要依赖于3种酶:能结合单链寡核苷酸引物的重组酶、单链DNA结合蛋白和DNA聚合酶。反应过程中不需要模板链的热变性,可以在恒定的低温条件下快速扩增DNA或者RNA。具有特异性强、灵敏度高、快速、操作简便、适用于现场快速诊断等特点,在动物疾病早期诊断、实地检测、进出口快速检疫等方面具有巨大的应用前景和市场。本文对RPA技术进行综述,以期为相关研究提供参考。
[Abstract]:It is of great significance for the prevention and control of animal epidemic disease, import and export quarantine, animal food safety and health to establish a rapid diagnosis of animal epidemic disease in China. Recombinant enzyme polymerase amplification (rpa) is a new nucleic acid amplification technique which is expected to replace PCR in recent years. The technique mainly depends on three enzymes: recombinant enzyme that binds single strand oligonucleotide primer, single strand DNA binding protein and DNA polymerase. The thermal denaturation of the template chain is not required during the reaction, and the DNA or RNA can be rapidly expanded at constant low temperature. It has the characteristics of strong specificity, high sensitivity, rapid operation, and is suitable for field rapid diagnosis. It has a great application prospect and market in the early diagnosis of animal diseases, field detection, import and export rapid quarantine and so on. In this paper, RPA technology is reviewed in order to provide reference for related research.
【作者单位】：中国农业科学院兰州兽医研究所/家畜疫病病原生物学国家重点实验室/甘肃省动物寄生虫病重点实验室;华南农业大学动物科学学院;吉林农业大学动物科学技术学院;
【基金】：国家科技支撑计划资助项目(2012BAD12B07) 国家公益性行业(农业)科研专项经费资助项目(201303037) 甘肃省创新研究群体计划资助项目(1210RJIA006)
【分类号】：S854.43

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