基于GC-MS代谢组学技术对内脂素调控雏鸡采食机制的研究

发布时间：2018-05-29 18:16

本文选题：Visfatin + 采食量　；参考：《畜牧兽医学报》2017年07期

【摘要】：旨在探索内脂素(Visfatin)调控家禽采食的分子机制,为完善家禽的食欲调控和能量平衡理论奠定基础。选取1日龄罗曼褐健康雏鸡20只,随机分为4个处理(C、L、M、H组)。常规饲粮饲养至10日龄进行下丘脑导管埋置,通过脑室注射技术对雏鸡下丘脑部位注射不同浓度的重组人内脂素(0、40、200、400ng)。脑室注射Visfatin60min后,记录各处理组累积采食量,并采集下丘脑组织,通过GC-MS系统进行代谢组学检测,建立PLS-DA模型,并将差异代谢物输入KEGG数据库进行代谢通路的构建。结果表明,脑室注射Visfatin 60min后,M组和H组的雏鸡累积采食量与C组相比均显著升高(P0.05)。在对雏鸡下丘脑进行GC-MS代谢组学分析后,处理组与对照组间共鉴定获得73个差异性代谢物,富集到51条通路上。脑室注射Visfatin后下丘脑产生的差异性代谢物与氨基酸、糖和脂代谢有着密切的联系,其中脂肪酸合成通路被显著富集。结果提示,中枢营养物质下降导致稳态发生改变可能是Visfatin发挥促食作用的主要机制。
[Abstract]:The aim of this study was to explore the molecular mechanism of visfatine (Visfatin) in regulating poultry intake, and to lay a foundation for improving the theory of appetite regulation and energy balance in poultry. A total of 20 1-day-old Roman brown healthy chicks were randomly divided into 4 groups. Hypothalamic catheterization was carried out by conventional diet for 10 days. Different concentrations of recombinant human lipopolipid were injected into the hypothalamus of chicks by intracerebroventricular injection (ICV). After intracerebroventricular injection of Visfatin60min, the cumulative feed intake was recorded and hypothalamus tissue was collected. The PLS-DA model was established by GC-MS system, and the differential metabolites were input into the KEGG database to construct the metabolic pathway. The results showed that the cumulative feed intake of chicks in group M and group H was significantly higher than that in group C after intraventricular injection of Visfatin 60min. After GC-MS metabonomics analysis of the hypothalamus of chicks, 73 different metabolites were identified between the treatment group and the control group and enriched in 51 pathways. The differential metabolites produced in hypothalamus after intracerebroventricular injection of Visfatin were closely related to amino acid, glucose and lipid metabolism, in which fatty acid synthesis pathway was significantly enriched. The results suggest that the change of homeostasis caused by the decrease of central nutrition may be the main mechanism of promoting food by Visfatin.
【作者单位】：河南农业大学牧医工程学院河南省家禽种质资源创新工程研究中心;
【基金】：国家自然科学基金(31372330) 教育部创新团队发展计划(IRT16R23) 河南省重大科技专项(151100110800)
【分类号】：S831

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