性成熟前猪睾丸组织中印记基因DNA甲基化状态分析

发布时间：2018-05-29 20:56

本文选题：DNA甲基化 + 印记基因　；参考：《浙江农业学报》2017年05期

【摘要】：为探求性成熟前猪睾丸中印记基因DNA甲基化状态,以1、2、3月龄三元杂交猪睾丸实质组织为材料,在利用Me DIP-seq(甲基化DNA免疫沉淀测序技术)建立性成熟前猪睾丸全基因组DNA甲基化图谱的基础上,比对得到了8个已证实的猪印记基因甲基化状态。结果显示,8个印记基因DIRAS3、IGF2、IGF2R、MEST、NAP1L5、NNAT、PEG10、PLAGL1,在性成熟前猪睾丸组织中的甲基化水平均不高,表现为"非甲基化"或"部分甲基化";NNAT、PLAGL1启动子甲基化水平在1~3月龄间保持增长,NAP1L5、IGF2R启动子甲基化水平在1~2月龄下降,2~3月龄增长,DIRAS3、IGF2、MEST、PEG10 promoter甲基化水平在1~2月龄增长,2~3月龄下降;图谱中仅比对到4个基因的gene body甲基化状态,IGF2R、MEST gene body甲基化水平在1~3月龄保持下降,PLAGL1、PEG10 gene body甲基化水平在1~2月龄增长,2~3月龄下降。推测这8个印记基因在性成熟前猪睾丸发育过程中均具有一定活性,且DNA甲基化对印记基因发挥一定的调控作用。
[Abstract]:In order to find out the methylation status of the imprinted gene DNA in porcine testicles before maturation, and using the three yuan 1,2,3 month old pig's testis parenchyma as material, 8 confirmed porcine imprinting genes a was compared by using Me DIP-seq (methylation DNA immunoprecipitation sequencing technology) to establish the complete genome DNA methylation map of the whole genome of porcine testis before sex maturation. The results showed that 8 imprinting genes, DIRAS3, IGF2, IGF2R, MEST, NAP1L5, NNAT, PEG10, PLAGL1, were not highly methylation levels in the testis tissues of pigs before sexual maturity, showing "non methylation" or "partial methylation"; NNAT, PLAGL1 starting Zi Jiaji level maintained growth during 1~3 months, NAP1L5, promoter methylation level was 2 month old decreased, 2~3 months of age increased, DIRAS3, IGF2, MEST, PEG10 promoter methylation level increased at the age of 1~2, and 2~3 month age decreased, and only the gene body methylation status of the 4 genes was compared. It is speculated that these 8 imprinted genes have certain activity in the development of pig testis before sexual maturation, and DNA methylation plays a regulatory role in imprinting genes.
【作者单位】：四川农业大学动物医学院;
【基金】：国家自然科学基金(31172379)
【分类号】：S828

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