注射促肾上腺皮质激素对母猪断奶后新生黄体的发育及类固醇合成的影响

发布时间：2018-05-29 21:45

本文选题：应激 + 外源促肾上腺皮质激素　；参考：《南京农业大学学报》2017年02期

【摘要】：[目的]本文旨在确定应激对母猪排卵后黄体组织的正常发育及其功能的影响。[方法]采用注射促肾上腺皮质激素(ACTH)的方式模拟应激状态,利用RT-q PCR和免疫组织化学法研究新生黄体的类固醇合成能力和组织发育状况。[结果]ACTH组母猪血浆中皮质醇含量明显高于对照组母猪,新生黄体内胆固醇侧链裂解酶P450(P450scc)和3β-羟基类固醇脱氢酶(3β-HSD)的表达量比对照组母猪明显下调;ACTH组母猪基质金属蛋白(MMP)和血管内皮生长因子(VEGFa)的表达量明显低于对照组母猪;组织学检测结果也显示ACTH组母猪的黄体组织填充率低于对照组母猪,而且黄体内血管密度相对较低。[结论]注射ACTH模拟应激,可诱导受试母猪血浆高皮质醇含量,而且能够显著降低新生黄体内主要孕酮合成酶的表达和黄体组织内部的血管新生。提示,应激能够影响母猪新生黄体的正常发育和功能。
[Abstract]:Objective: to determine the effect of stress on the normal development and function of luteal tissue after ovulation in sows. [methods] the stress state was simulated by injecting adrenocorticotropic hormone (ACTH), and the steroid synthesis ability and tissue development of the corpus luteum were studied by RT-q PCR and immunohistochemistry. [results] the content of cortisol in plasma of ACTH group was significantly higher than that of control group. The expression of cholesterol side chain lyase P450 SCC and 3 尾 -hydroxysteroid dehydrogenase 3 尾 -HSDin in newborn yellow was significantly lower than that in control sows. The expression of matrix metalloprotein (MMPP) and vascular endothelial growth factor (VEGF) in sows of ACTH group was significantly lower than that in control sows. Histological examination also showed that the filling rate of luteal tissue in ACTH sows was lower than that in control sows, and the vessel density in yellow sows was relatively low. [conclusion] injection of ACTH to mimic stress can induce high cortisol content in plasma, and can significantly reduce the expression of progesterone synthase and angiogenesis in corpus luteum. The results suggest that stress can affect the normal development and function of newborn corpus luteum in sows.
【作者单位】：南京农业大学动物医学院;南京农业大学动物科技学院;
【基金】：国家重点基础研究计划(973计划)资助项目(2014CB138502)
【分类号】：S828

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