CD14基因在不同发育阶段梅山猪各组织中的表达趋势和差异分析

发布时间：2018-05-30 22:14

本文选题：CD基因 + 猪　；参考：《中国畜牧杂志》2017年04期

【摘要】：为分析CD14基因在猪各组织中的发育表达差异情况,进一步探讨该基因的免疫调控机制,实验利用实时荧光定量PCR比较分析CD14基因在7个不同发育阶段梅山猪的12个组织中的表达水平。结果表明:CD14基因在不同发育阶段梅山猪的12个组织中均有表达,且在不同阶段呈现相对一致的表达规律,在肝中呈现极高的表达水平,在脾、肺、肠系膜淋巴结等组织中呈现中等的表达水平,在其他组织中呈现较低的表达水平。发育性表达分析显示,CD14基因在肝脏中从初生到成年一直保持较高的表达水平;在免疫组织(脾、肺、肾和胸腺)、肠道组织(十二指肠、空肠和回肠)和胃组织中,CD14基因的表达水平从初生起呈上升趋势,14或21日龄表达水平最高,之后呈下降趋势并一直保持较低的表达水平,在肠系膜淋巴结中,初生仔猪CD14基因的表达水平最高,之后显著下降并维持较低的表达水平。由此推测,CD14基因的表达水平变化趋势与仔猪自身免疫发育具有相对的一致性,且与仔猪抵抗开食期和断奶期的应激有着密切的联系。
[Abstract]:In order to analyze the developmental and expression differences of CD14 gene in pig tissues, and to further explore the immunological regulation mechanism of the gene, The expression of CD14 gene in 12 tissues of 7 different developmental stages of Meishan pig was analyzed by real-time fluorescence quantitative PCR. The results showed that the gene was expressed in 12 tissues of Meishan pig at different developmental stages, and showed a relatively consistent expression pattern in different stages, and a very high expression level in liver, spleen, lung, spleen and lung. Mesenteric lymph nodes and other tissues showed moderate expression level and low expression level in other tissues. Developmental expression analysis showed that the expression of CD14 gene remained high in the liver from birth to adulthood, and in the immune tissues (spleen, lung, kidney and thymus), intestinal tissue (duodenum, duodenum). The expression level of CD14 gene in jejunum and ileum) and gastric tissues increased from the beginning of life to the highest level at 14 or 21 days of age, then decreased and maintained a low expression level, and in mesenteric lymph nodes, the expression level of CD14 gene in the mesenteric lymph nodes was lower than that in the mesenteric lymph nodes. The expression level of CD14 gene in newborn piglets was the highest, then decreased significantly and maintained a low level. It is inferred that the change trend of CD14 gene expression level is relatively consistent with the autoimmune development of piglets and is closely related to the resistance of piglets to stress during feeding and weaning.
【作者单位】：扬州大学江苏省动物遗传繁育与分子设计重点实验室;江苏省太仓市畜牧兽医站;
【基金】：江苏省科技支撑计划重点项目(BE2014357) 苏州市科技局攻关项目(SNG201628)
【分类号】：S828.2

【参考文献】